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1Academic Journal
المؤلفون: Thomas Démoulins, Thatcha Yimthin, Dorothea Lindtke, Lukas Eggerschwiler, Raphael Siegenthaler, Fabien Labroussaa, Joerg Jores
المصدر: Veterinary Research, Vol 55, Iss 1, Pp 1-22 (2024)
مصطلحات موضوعية: Mycoplasmopsis bovis, multiparameter flow cytometry assay, multiplex immunoassay, immune cell subsets, high fever temperature, Veterinary medicine, SF600-1100
وصف الملف: electronic resource
Relation: https://doaj.org/toc/1297-9716
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2Academic Journal
المؤلفون: Xiaobo Wang, Wenlong Xu, Zengyiyi Wang, Qian Yu, Li Yuan, Yihang Liu, Jinpeng Sang, Wei Li, Sanyong Zhu, Wei Jiang, Zengxia Li, Wen Zhang, Yongjun Dang
مصطلحات موضوعية: Biophysics, Biochemistry, Cell Biology, Genetics, Molecular Biology, Biotechnology, Immunology, Cancer, Space Science, Environmental Sciences not elsewhere classified, Biological Sciences not elsewhere classified, screen bioactive compounds, potential immunotherapeutic agent, flow cytometry assay, l1 inhibitory activity, l1 expression, tumor cells, sokotrasterol sulfate, signal transducer, pdl1 <, multiple cancers, may act, immunological role, findings highlight, efficiently suppress, efficient non, downstream activation, cell killing
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3Academic Journal
المؤلفون: Thomas Démoulins, Thatcha Yimthin, Dorothea Lindtke, Lukas Eggerschwiler, Raphael Siegenthaler, Fabien Labroussaa, Joerg Jores
مصطلحات موضوعية: Immunology, Mycoplasmopsis bovis, multiparameter flow cytometry assay, multiplex immunoassay, immune cell subsets, high fever temperature
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4Academic Journal
المؤلفون: Débora Zini Baldasso, João Antônio Guizzo, Cláudia Cerutti Dazzi, Gabriela Carolina Paraboni Frandoloso, César Feronato, Stephan von Berg, Roberto Maurício Carvalho Guedes, Heather Lynne Wilson, Luiz Carlos Kreutz, Rafael Frandoloso
المصدر: Frontiers in Immunology, Vol 14 (2023)
مصطلحات موضوعية: Lawsonia intracellularis, Ileitis, pig, diagnostic test, flow cytometry assay, serological antibody detection, Immunologic diseases. Allergy, RC581-607
وصف الملف: electronic resource
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5Academic Journal
المؤلفون: Hiroto EGAWA, Hitomi MIURA, Kazuhiro IWADOH, Mayu SASANO, Mineko YASUO, Ryuji FUJITA, Tsutomu ISHIZUKA, Yuri KOBAYASHI, 三浦 ひとみ, 安尾 美年子, 小林 悠梨, 岩藤 和広, 江川 裕人, 石塚 敏, 笹野 まゆ, 藤田 龍司
المصدر: 医学検査 / Japanese Journal of Medical Technology. 2022, 71(2):222
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6Academic Journal
المؤلفون: Mahnaz Maddahfar (10828649), Shihui Wen (1624444), Seyed Mostafa Hosseinpour Mashkani (10828652), Lin Zhang (8926), Olga Shimoni (1292151), Martina Stenzel (2723158), Jiajia Zhou (696159), Barbara Fazekas de St Groth (581014), Dayong Jin (318266)
مصطلحات موضوعية: Biophysics, Biochemistry, Pharmacology, Biotechnology, Cancer, Cardiology, Chemical Sciences not elsewhere classified, 13 OEGMEA units, nanoparticle, methyl ether acrylate, B 220 biomarkers, POEGMEA chain, bioactive lanthanide-doped nanoconj., glycol, 55 OEGMEA units, efficiency, mass cytometry application, antibody, flow cytometry assay, MAEP, 7 monoacryloxyethyl phosphate, design diblock copolymers
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7Academic Journal
المؤلفون: Xin Yang, Zuoping He, Guoxia Zhang, Jinhui Lu, Hui Zhang, Hui Ren, Yanjun Tian, Heng Yang, Chuangfu Chen, Linhai Li, Yongshui Fu, Jean-Pierre Allain, Chengyao Li, Wenjing Wang
المصدر: Frontiers in Cellular and Infection Microbiology, Vol 10 (2020)
مصطلحات موضوعية: brucellosis, Brucella, Omp25, mAb, flow cytometry assay, diagnosis, Microbiology, QR1-502
وصف الملف: electronic resource
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8Academic Journal
المؤلفون: Pauline Franz (9445992), Alexander Bürkle (1296921), Peter Wick (435597), Cordula Hirsch (1350444)
مصطلحات موضوعية: Biochemistry, Molecular Biology, Biotechnology, Cancer, Science Policy, Space Science, Biological Sciences not elsewhere classified, Chemical Sciences not elsewhere classified, data reliability, flow cytometry-based MN, scalable, ENM-induced, Reliable Nanomaterial Genotoxicity ., flow cytometry-based micronuclei, interference, ENM, flow cytometric readout, Exploring Flow Cytometry-Based Micr., genotoxicity, Comet assay, assessment, DNA, vivo nanogenotoxicological data, TiO 2, nanogenotoxicity, SiO 2, MN flow cytometry assay, nanomaterial, cell gel electrophoresis
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9Academic Journal
المؤلفون: Yang, Xin, He, Zuoping, Zhang, Guoxia, Lu, Jinhui, Zhang, Hui, Ren, Hui, Tian, Yanjun, Yang, Heng, Chen, Chuangfu, Li, Linhai, Fu, Yongshui, Allain, Jean-Pierre, Li, Chengyao, Wang, Wenjing
مصطلحات موضوعية: Brucella, Omp25, brucellosis, diagnosis, flow cytometry assay, mAb, Animals, Antibodies, Bacterial, Monoclonal, Bacterial Outer Membrane Proteins, Enzyme-Linked Immunosorbent Assay, Humans, Leukocytes, Mononuclear, Mice
وصف الملف: application/zip; application/pdf; text/xml
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10Academic Journal
المؤلفون: Rojas Vasquez, Olga Lucía
المساهمون: Pontificia Universidad Javeriana. Facultad de Medicina. Instituto de Genética Humana
المصدر: Virology; Vol. 380 Núm. 2 (2008)
مصطلحات موضوعية: Rotavirus, Memory B cell, Limiting dilution assay, Flow cytometry assay
وصف الملف: Papel / Electrónico; application/pdf
Relation: https://www-sciencedirect-com.ezproxy.javeriana.edu.co/science/article/pii/S0042682208005163; 0042-6822 / 1096-0341 (Electrónico); http://hdl.handle.net/10554/45813; https://doi.org/10.1016/j.virol.2008.08.004
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11Academic Journal
المؤلفون: Yeu-Chin Chen, Chih-Yuan Lin, Chien-Sung Tsai
المصدر: Journal of the Formosan Medical Association, Vol 114, Iss 10, Pp 981-987 (2015)
مصطلحات موضوعية: anti-heparin/platelet factor 4 antibodies, cardiopulmonary bypass surgery, flow cytometry assay, heparin-induced thrombocytopenia, Medicine (General), R5-920
وصف الملف: electronic resource
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12Dissertation/ Thesis
المؤلفون: Tavares, Lucas Alves
Thesis Advisors: Silva, Luis Lamberti Pinto da
مصطلحات موضوعية: 2 depletion, acts in the CD4 down-regulation induced by Nef. These studies contribute to a better understanding on the molecular mechanisms involved in Nef activit, although not need charging ubiquitination, and it is estimated that there are currently at least 36.9 million people infected with the virus. During the replication cycle, and only the AP-1 variant comprising ?2, another subunit of AP-1, AP-1, AP-1 subunits. By pull-down technique, AP-2, AP-2 and AP-3 are potentially heterogeneous due to the existence of multiple subunits isoforms encoded by different genes. However, AP-3 complexes and Nef does not appear to interact with AP-4 and AP-5 subunits. However, being an important protein in the endo-lysosomal system. Furthermore, but also seem to compose AP-1 complex with distinct cell functions, but not ?1, but not ?1 depletion, but the molecular mechanisms are still incompletely understood. Nef forms a tripartite complex with the cytosolic tail of the CD4 and adapter protein, by flow cytometry assay, compromises the reduction of surface CD4 levels induced by Nef. Immunofluorescence microscopy analysis also revealed that ?2 depletion impairs the red, compromising the efficient CD4 degradation by Nef. Moreover, depends on the proteins from ESCRTs (Endosomal Sorting Complexes Required for Transport) machinery and the action of Alix, an accessory protein ESCRT, ESCRT, HIV promotes several changes in the physiology of the host cell to promote their survival and enhance replication. The fast progression of HIV-1 in hu, HIV-1, inducing CD4 internalization and lysosome degradation. Previous research has demonstrated that CD4 target to lysosomes by Nef involves targeting of th, internalized CD4 accumulates in enlarged HRS-GFP positive endosomes, is required in Nef-mediated targeting of CD4 to lysosomes and the ?2 participation in this process is conserved by Nef from different viral strains. F, MVB, Nef, not ?1-adaptin, one is the most important is the down-regulation of proteins from the immune response, our data from immunoblots indicated that ?2- adaptin, regulação negativa de CD4, resulted in decreased cellular levels of ?1 and ?2 and, resulting in CD4 accumulation in primary endosomes. Knockdown of ?1A, such as the CD4 receptor. It is known that this action causes CD4 degradation in lysosome, the results indicate that ?-adaptins isoforms not only have different functions, the results indicate that ?2-adaptin is a molecule that is essential for CD4 targeting by Nef to ESCRT/MVB pathway, The Human Immunodeficiency Virus (HIV) is the etiologic agent of Acquired Immunodeficiency Syndrome (AIDS). AIDS is a disease which has a global distr, the role of Nef interaction with AP-1 or AP-3 in CD4 down-regulation is poorly understood. Furthermore, there are few studies to demonstrate if the different combinations of APs isoforms are form and if they have distinct functional properties. This stud, this study aimed to characterize the involvement of AP-1 complex in the down-regulation of CD4 by Nef HIV-1 through the functional study of the two is, this work contributes with the understanding of primordial process regulation on intracellular trafficking of transmembrane proteins., upon artificially stabilizing ESCRT-I in early endosomes, via overexpression of HRS, we showed that Nef is able to interact with ?2. In addition, where co-localize with ?2. Together, which may also help to improve the understanding of the HIV pathogenesis and the related syndrome. In addition, y1-adaptina, y2-adaptina
وصف الملف: application/pdf
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13
المؤلفون: Hannah-Lou Schilling, Gunther Glehr, Michael Kapinsky, Norbert Ahrens, Paloma Riquelme, Laura Cordero, Florian Bitterer, Hans J. Schlitt, Edward K. Geissler, Sebastian Haferkamp, James A. Hutchinson, Katharina Kronenberg
المصدر: Frontiers in Immunology
Frontiers in Immunology, Vol 12 (2021)مصطلحات موضوعية: Adult, CD4-Positive T-Lymphocytes, Male, CD14, T cell, Immunology, 610 Medizin, Flow cytometry, Memory T Cells, effector memory T cells, medicine, Humans, Immunology and Allergy, Immune Checkpoint Inhibitors, Melanoma, Original Research, Aged, Aged, 80 and over, Hepatitis, medicine.diagnostic_test, business.industry, flow cytometry, Monocyte, prediction, Middle Aged, RC581-607, medicine.disease, assay validation, Immune checkpoint, medicine.anatomical_structure, flow cytometry, assay validation, immune checkpoint inhibition, immune-related adverse events, prediction, effector memory T cells, monocytes, biomarker, Cancer research, biomarker, immune checkpoint inhibition, immune-related adverse events, Biomarker (medicine), Female, Seasons, Immunologic diseases. Allergy, monocytes, business, Memory T cell
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14
المؤلفون: Sang Jun Ha, Young Ho Ban
المصدر: Immune Network
مصطلحات موضوعية: 0301 basic medicine, Immunology, Virus plaque assay, Lymphocytic choriomeningitis, Virus, Flow cytometry, 03 medical and health sciences, In vivo, medicine, Immunology and Allergy, Lymphocytic choriomeningitis virus, Nucleoprotein, Virus quantification, medicine.diagnostic_test, biology, Chemistry, Semi-functional quantitative flow cytometry assay, medicine.disease, Virology, 030104 developmental biology, Infectious Diseases, Real-time polymerase chain reaction, biology.protein, Original Article, Antibody
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15
المؤلفون: Yang Liu, Ming Du, Genyi Zhang
المصدر: Toxicology Reports, Vol 1, Iss C, Pp 1076-1086 (2014)
Toxicology Reportsمصطلحات موضوعية: Aflatoxin B1, ST, sterigmatocystin, Membrane permeability, Health, Toxicology and Mutagenesis, Sterigmatocystin, Cell, Combinative toxicity, Apoptosis, Mitochondrion, Biology, Toxicology, Article, PI, propidium iodide, FCM, flow cytometry assay, chemistry.chemical_compound, lcsh:RA1190-1270, medicine, lcsh:Toxicology. Poisons, MMP, mitochondrial membrane permeability, Cell growth, ATCC, American type culture collection, Cell cycle, PCA, principle component analysis, Molecular biology, medicine.anatomical_structure, chemistry, Biochemistry, SRB, sulforhodamine B, AFB1, aflatoxin B1, Intracellular
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16
المؤلفون: Sabrina Solouki, Saikat Boliar, Kaley M Wilburn, David W. Gludish, Kondwani C. Jambo, Henry C. Mwandumba, David G. Russell
المصدر: ResearcherID
Retrovirologyمصطلحات موضوعية: 0301 basic medicine, In situ, Transcription, Genetic, 030106 microbiology, wc_503_6, Human immunodeficiency virus (HIV), wc_503, Genome, Viral, Biology, Real-Time Polymerase Chain Reaction, medicine.disease_cause, Genome, Flow cytometry, HIV cell-associated RNA, 03 medical and health sciences, qu_58.7, Proviruses, FISH:FLOW, Transcription (biology), Cell Line, Tumor, Virology, Correspondence, HIV proviral loss, FISH:FACS, medicine, Humans, In Situ Hybridization, Fluorescence, HIV proviral silencing, Messenger RNA, medicine.diagnostic_test, HIV flow cytometry assay, Precursor Cell Lymphoblastic Leukemia-Lymphoma, Provirus, Flow Cytometry, Molecular biology, HIV RNA in situ hybridization, 3. Good health, 030104 developmental biology, Infectious Diseases, DNA, Viral, Reservoir quantitation, HIV-1, biology.protein, RNA, Viral, 8E5, Antibody, Transcription Factors
وصف الملف: application/pdf
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17Dissertation/ Thesis
المؤلفون: Lucas Alves Tavares
Thesis Advisors: Luis Lamberti Pinto da Silva, Renato Santana de Aguiar, Eurico de Arruda Neto
المصدر: Biblioteca Digital de Teses e Dissertações da USPUniversidade de São PauloUSP.
مصطلحات موضوعية: AP-1, ESCRT, HIV-1, MVB, Nef, regulação negativa de CD4, y1-adaptina, y2-adaptina, 2 depletion, acts in the CD4 down-regulation induced by Nef. These studies contribute to a better understanding on the molecular mechanisms involved in Nef activit, although not need charging ubiquitination, and it is estimated that there are currently at least 36.9 million people infected with the virus. During the replication cycle, and only the AP-1 variant comprising ?2, another subunit of AP-1, AP-1 subunits. By pull-down technique, AP-2, AP-2 and AP-3 are potentially heterogeneous due to the existence of multiple subunits isoforms encoded by different genes. However, AP-3 complexes and Nef does not appear to interact with AP-4 and AP-5 subunits. However, being an important protein in the endo-lysosomal system. Furthermore, but also seem to compose AP-1 complex with distinct cell functions, but not ?1, but not ?1 depletion, but the molecular mechanisms are still incompletely understood. Nef forms a tripartite complex with the cytosolic tail of the CD4 and adapter protein, by flow cytometry assay, compromises the reduction of surface CD4 levels induced by Nef. Immunofluorescence microscopy analysis also revealed that ?2 depletion impairs the red, compromising the efficient CD4 degradation by Nef. Moreover, depends on the proteins from ESCRTs (Endosomal Sorting Complexes Required for Transport) machinery and the action of Alix, an accessory protein ESCRT, HIV promotes several changes in the physiology of the host cell to promote their survival and enhance replication. The fast progression of HIV-1 in hu, inducing CD4 internalization and lysosome degradation. Previous research has demonstrated that CD4 target to lysosomes by Nef involves targeting of th, internalized CD4 accumulates in enlarged HRS-GFP positive endosomes, is required in Nef-mediated targeting of CD4 to lysosomes and the ?2 participation in this process is conserved by Nef from different viral strains. F, not ?1-adaptin, one is the most important is the down-regulation of proteins from the immune response, our data from immunoblots indicated that ?2- adaptin, resulted in decreased cellular levels of ?1 and ?2 and, resulting in CD4 accumulation in primary endosomes. Knockdown of ?1A, such as the CD4 receptor. It is known that this action causes CD4 degradation in lysosome, the results indicate that ?-adaptins isoforms not only have different functions, the results indicate that ?2-adaptin is a molecule that is essential for CD4 targeting by Nef to ESCRT/MVB pathway, The Human Immunodeficiency Virus (HIV) is the etiologic agent of Acquired Immunodeficiency Syndrome (AIDS). AIDS is a disease which has a global distr, the role of Nef interaction with AP-1 or AP-3 in CD4 down-regulation is poorly understood. Furthermore, there are few studies to demonstrate if the different combinations of APs isoforms are form and if they have distinct functional properties. This stud, this study aimed to characterize the involvement of AP-1 complex in the down-regulation of CD4 by Nef HIV-1 through the functional study of the two is, this work contributes with the understanding of primordial process regulation on intracellular trafficking of transmembrane proteins., upon artificially stabilizing ESCRT-I in early endosomes, via overexpression of HRS, we showed that Nef is able to interact with ?2. In addition, where co-localize with ?2. Together, which may also help to improve the understanding of the HIV pathogenesis and the related syndrome. In addition
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18
المؤلفون: Daniel F. Alonso, Marina Albertó, Héctor Adrián Cuello, Analía Pesce, Valeria Inés Segatori, Mariano R. Gabri
المصدر: BioDrugs : clinical immunotherapeutics, biopharmaceuticals and gene therapy. 30(3)
مصطلحات موضوعية: 0301 basic medicine, Patent Expiration, Farmacología y Farmacia, CIENCIAS MÉDICAS Y DE LA SALUD, L-Lactate dehydrogenase, Pharmacology, Cell Line, Adcc Activity, 03 medical and health sciences, 0302 clinical medicine, Biosimilar Pharmaceuticals, Flow Cytometry Assay, Medicine, Humans, Pharmacology (medical), Polymorphism, Genetic, biology, Dose-Response Relationship, Drug, L-Lactate Dehydrogenase, business.industry, Receptors, IgG, Antibody-Dependent Cell Cytotoxicity, Biosimilar, Reference Product, General Medicine, Antigens, CD20, Virology, Reference product, Medicina Básica, 030104 developmental biology, Cell killing, 030220 oncology & carcinogenesis, Antibody-dependent cell cytotoxicity, biology.protein, Rituximab, Antibody, business, Cd20 Positive Cell, Biotechnology, medicine.drug
وصف الملف: application/pdf
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19
المؤلفون: Jean Cohen, Annie Charpilienne, Manuel A. Franco, Agathe Ogier, Pierre Pothier, Evelyne Kohli
المساهمون: Centre Hospitalier Universitaire de Dijon - Hôpital François Mitterrand (CHU Dijon), Instituto de Genética Humana, Pontificia Universidad Javeriana (PUJ), Virologie moléculaire et structurale (VMS), Institut National de la Recherche Agronomique (INRA)-Centre National de la Recherche Scientifique (CNRS)
المصدر: European Journal of Immunology
European Journal of Immunology, Wiley-VCH Verlag, 2005, 35 (7), pp.2122-2130. ⟨10.1002/eji.200526059⟩مصطلحات موضوعية: [SDV]Life Sciences [q-bio], Lymphocyte Activation, Feces, Mice, 0302 clinical medicine, Immunophenotyping, Immunology and Allergy, Mesenteric lymph nodes, Intestinal Mucosa, Antigens, Viral, B-Lymphocytes, Mice, Inbred BALB C, 0303 health sciences, biology, medicine.diagnostic_test, 3. Good health, medicine.anatomical_structure, Vaccines, Subunit, Antibody, Bacterial Toxins, Immunology, Spleen, FLOW CYTOMETRY ASSAY, Antibodies, Flow cytometry, Viral Proteins, 03 medical and health sciences, Antigen, medicine, Animals, INTRANASAL IMMUNIZATION, Administration, Intranasal, B cell, 030304 developmental biology, Virion, Viral Vaccines, Immunoglobulin D, Molecular biology, Nasal Mucosa, B CELLS, Mutation, ALPHA 4 BETA 7, biology.protein, Capsid Proteins, Lymph Nodes, ROTAVIRUS, 030215 immunology, Homing (hematopoietic)
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20
المؤلفون: Wilburn, Kaley, Mwandumba, Henry, Jambo, Kondwani, Boliar, Saikat, Solouki, Sabrina, Russell, David, Gludish, David