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المؤلفون: Forte, Elvira
المساهمون: Giacomello, Alessandro
مصطلحات موضوعية: Intra-peritoneal injection and oral gavage on mice, organs and embryos harvesting. - Preparation of primary cultures (cardiac explants) and growth of cardiac progenitor cells and mESCs. - Langendorff preparation to disperse cells from the heart. - Cloning and transfection of cells in culture. - Cellular characterization by Immunohistochemistry, Immunofluorescence and FACS sorting. - Proteins extraction and Western Blotting. - RNA and DNA extraction and purification. - PCR (Polymerase Chain Reaction)/ RT-PCR (Sybr Green and Taqman). - Bacterial cultures and bacterial transformation Good knowledge of Bioinformatic tools. Use of ImageJ, OligoExplorer, BLAST. Basic knowledge of CorelDraw, CLUSTALW, Rasmol v.2.6, Swiss Pdb Viewer, Clone, Gene Runner, EMT, cardiac stem cells origin and differentiation, epicardium, cardiomyocytes, Cardiac stem cells