المؤلفون: LI Yuan, ZHONG Haiying, DONG Shifang

المصدر: 陆军军医大学学报, Vol 46, Iss 13, Pp 1512-1522 (2024)

مصطلحات موضوعية: bone derived mesenchymal stem cells, bmp2, osteogenic differentiation, aging, cluster of differentiation 51, Medicine (General), R5-920

وصف الملف: electronic resource

Relation: https://aammt.tmmu.edu.cn/html/202310032.htm; https://doaj.org/toc/2097-0927

URL الوصول: https://doaj.org/article/3dfdc565eaf548f0b7d6dbd5246ccd3d

المؤلفون: Zhen Zhou, Changbin Zhao, Bolin Cai, Manting Ma, Shaofen Kong, Jing Zhang, Xiquan Zhang, Qinghua Nie

المصدر: Animals; Volume 12; Issue 12; Pages: 1531

مصطلحات موضوعية: broiler, bone-derived mesenchymal stem cells, 5-azacytidine, dexamethasone, hydrocortisone, myogenic determination and differentiation

جغرافية الموضوع: agris

وصف الملف: application/pdf

Relation: https://dx.doi.org/10.3390/ani12121531

الاتاحة: https://doi.org/10.3390/ani12121531

المؤلفون: O Evrova, J Buschmann

المصدر: European Cells & Materials, Vol 34, Pp 15-39 (2017)

مصطلحات موضوعية: the range of bioactive components in dentine and specific biological effects on bone-derived mesenchymal stem cells (MSCs) in humans are less well understood. The aims of this study were to further elucidate the biological response of MSCs to demineralised dentine matrix (DDM) in enhancing wound repair responses and ascertain key contributing components. Dentine was obtained from human teeth and DDM proteins solubilised with ethylenediaminetetraacetic acid (EDTA). Bone marrow derived MSCs were commercially obtained. Cells with a more immature phenotype were then selected by preferential fibronectin adhesion (FN-BMMSCs) for use in subsequent in vitro assays. DDM at 10 µg/mL reduced cell expansion, attenuated apoptosis and was the minimal concentration capable of inducing osteoblastic differentiation. Enzyme-linked immunosorbent assay (ELISA) quantification of growth factors indicated physiological levels produced the above responses, transforming growth factor β (TGF-β1) was predominant (15.6 ng/mg DDM), with relatively lower concentrations of BMP-2, FGF, VEGF and PDGF (6.2-4.7 ng/mg DDM). Fractionation of growth factors from other DDM components by heparin affinity chromatography diminished osteogenic responses. Depletion of biglycan from DDM also attenuated osteogenic potency, which was partially rescued by the isolated biglycan. Decorin depletion from DDM had no influence on osteogenic potency. Collectively, these results demonstrate the potential of DDM for the delivery of physiological levels of growth factors for bone repair processes, Mesenchymal stem cells, dentine, growth factors, biglycan, decorin, cell expansion, osteoblast differentiation, migration, apoptosis, Diseases of the musculoskeletal system, RC925-935, Orthopedic surgery, RD701-811

وصف الملف: electronic resource

Relation: http://www.ecmjournal.org/papers/vol034/pdf/v034a02.pdf; https://doaj.org/toc/1473-2262

URL الوصول: https://doaj.org/article/5470f7650d56439dad0fee05808bc9d0

المؤلفون: Olivera Evrova, Johanna Buschmann

المساهمون: University of Zurich

المصدر: European Cells & Materials, Vol 34, Pp 15-39 (2017)

مصطلحات موضوعية: 0301 basic medicine, VEGF and PDGF (6.2-4.7 ng/mg DDM). Fractionation of growth factors from other DDM components by heparin affinity chromatography diminished osteogenic responses. Depletion of biglycan from DDM also attenuated osteogenic potency, 1303 Biochemistry, lcsh:Diseases of the musculoskeletal system, Angiogenesis, these results demonstrate the potential of DDM for the delivery of physiological levels of growth factors for bone repair processes, Becaplermin, Bioinformatics, with relatively lower concentrations of BMP-2, migration, dentine, 1307 Cell Biology, Tendons, Drug Delivery Systems, FGF, 10266 Clinic for Reconstructive Surgery, decorin, biology, apoptosis, Proto-Oncogene Proteins c-sis, osteoblast differentiation, Drug delivery, the range of bioactive components in dentine and specific biological effects on bone-derived mesenchymal stem cells (MSCs) in humans are less well understood. The aims of this study were to further elucidate the biological response of MSCs to demineralised dentine matrix (DDM) in enhancing wound repair responses and ascertain key contributing components. Dentine was obtained from human teeth and DDM proteins solubilised with ethylenediaminetetraacetic acid (EDTA). Bone marrow derived MSCs were commercially obtained. Cells with a more immature phenotype were then selected by preferential fibronectin adhesion (FN-BMMSCs) for use in subsequent in vitro assays. DDM at 10 µg/mL reduced cell expansion, Platelet-derived growth factor receptor, cell expansion, medicine.drug, Signal Transduction, lcsh:Surgery, 2204 Biomedical Engineering, Context (language use), 610 Medicine & health, Models, Biological, Fibrin, 03 medical and health sciences, In vivo, growth factors, medicine, Animals, Humans, Wound Healing, 1502 Bioengineering, business.industry, 2502 Biomaterials, lcsh:RD1-811, biglycan, In vitro, 030104 developmental biology, attenuated apoptosis and was the minimal concentration capable of inducing osteoblastic differentiation. Enzyme-linked immunosorbent assay (ELISA) quantification of growth factors indicated physiological levels produced the above responses, transforming growth factor β (TGF-β1) was predominant (15.6 ng/mg DDM), biology.protein, Mesenchymal stem cells, lcsh:RC925-935, business, which was partially rescued by the isolated biglycan. Decorin depletion from DDM had no influence on osteogenic potency. Collectively

URL الوصول: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::87896af71997b06e2b0ea7448a8f8326
http://www.ecmjournal.org/papers/vol034/pdf/v034a02.pdf

المؤلفون: Huayu Wu, Danqing Lei, Jinmin Zhao, Zhenhui Lu, Zhikang Miao, Yi He, Li Zheng, Luo Shixing

المصدر: Molecular Medicine Reports

مصطلحات موضوعية: Male, 0301 basic medicine, Cancer Research, Cell Survival, medicine.medical_treatment, Fluorescent Antibody Technique, Cell morphology, Biochemistry, nerve growth factor, Mice, 03 medical and health sciences, chemistry.chemical_compound, 0302 clinical medicine, chondrogenesis, Genetics, medicine, Animals, Nerve Growth Factors, Propidium iodide, Molecular Biology, Aggrecan, Cell Proliferation, Elapid Venoms, Cell growth, Gene Expression Profiling, Growth factor, Mesenchymal stem cell, Gene Expression Regulation, Developmental, Cell Differentiation, Mesenchymal Stem Cells, Articles, Chondrogenesis, Immunohistochemistry, Recombinant Proteins, Rats, bone-derived mesenchymal stem cells, Cell biology, 030104 developmental biology, Nerve growth factor, Chinese cobra venom, Oncology, chemistry, recombinant murine β-nerve growth factor, 030220 oncology & carcinogenesis, Molecular Medicine, Collagen, Corrigendum, Biomarkers

URL الوصول: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::046a71d8dccd7f1e0d90a198de7a4a69
https://doi.org/10.3892/mmr.2018.9307

المؤلفون: Barcellos de Souza, P, Comito, Giuseppina, Pons Segura, C, Taddei, Maria Letizia, Gori, Valentina, Becherucci, V, Bambi, F, Margheri, Francesca, Laurenzana, Anna, Del Rosso, Mario, Chiarugi, Paola

مصطلحات موضوعية: Bone-derived mesenchymal stem cells, Carcinoma-associated fibroblast, Carcinoma-associated macrophages, Prostate cancer, TGF-β, Tumor microenvironment

URL الوصول: https://explore.openaire.eu/search/publication?articleId=od_______310::d2617c783db6f90231f20b3d2ac2d387
http://hdl.handle.net/2158/1059507