المؤلفون: Jung-Il Cho, Emmanuel Guiderdoni, Jeong Eun Park, Ki-Hong Jung, Hye Rang Park, Cong Danh Nguyen, Sunok Moon, Jin Hoe Huh, Jong-Seong Jeon, Sang-Kyu Lee, Hyun-Bi Kim

المساهمون: Kyung Hee University (KHU), Seoul National University [Seoul] (SNU), Amélioration génétique et adaptation des plantes méditerranéennes et tropicales (UMR AGAP), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Département Systèmes Biologiques (Cirad-BIOS), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad), Next Generation BioGreen 21 Program of the Rural Development Administration of Korea (PJ013172012019), Mid-Career Researcher Program of the National Research Foundation of Korea (NRF-2017R1A2B4009687)

المصدر: Journal of Experimental Botany
Journal of Experimental Botany, Oxford University Press (OUP), 2019, 71 (1), pp.116-125. ⟨10.1093/jxb/erz436⟩

مصطلحات موضوعية: 0106 biological sciences, Infertilité mâle, the mutant pollen was unable to compete successfully against wild-type pollen. Expression of the catalytically inactive OsHXK5-G113D did not rescue the hxk5 male-sterile phenotype, Physiology, Starch, Stamen, Plant Science, medicine.disease_cause, 01 natural sciences, chemistry.chemical_compound, Hexokinase, produced via anther culture, and additional homozygous hxk5-2, Tube du pollen, Pollen maturation, Plant Proteins, 0303 health sciences, F63 - Physiologie végétale - Reproduction, food and beverages, Cell biology, Germination, Pollen, Pollen tube, Amidon, Sterility, There is little known about the function of rice hexokinases (HXKs) in planta. We characterized hxk5-1, F60 - Physiologie et biochimie végétale, Oryza sativa, Biology, rather than its role in sugar sensing and signaling. Our results demonstrate that OsHXK5 contributes to a large portion of the hexokinase activity necessary for the starch utilization pathway during pollen germination and tube growth, Germination du pollen, 03 medical and health sciences, a Tos17 mutant of OsHXK5 that is up-regulated in maturing pollen, medicine, otorhinolaryngologic diseases, [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, indicating that its catalytic function was responsible for pollen fertility, although they were sufficient to produce some viable seed. However, 030304 developmental biology, a stage when starch accumulates. Progeny analysis of self-pollinated heterozygotes of hxk5-1 and reciprocal crosses between the wild-type and heterozygotes revealed that loss of HXK5 causes male sterility. Homozygous hxk5-1, Base Sequence, hxk5-3 and hxk5-4 lines created by CRISPR/Cas9 confirmed the male-sterile phenotype. In vitro pollen germination ability and in vivo pollen tube growth rate were significantly reduced in the hxk5 mutant pollen. Biochemical analysis of anthers with the mutant pollen revealed significantly reduced hexokinase activity and starch content, Oryza, Fertility, chemistry, as well as for starch biosynthesis during pollen maturation, 010606 plant biology & botany

وصف الملف: text

URL الوصول: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d756591348ca6963458a58aa72340510
http://agritrop.cirad.fr/599434/

المؤلفون: Lee, Sang-Kyu, Kim, Hyunbi, Cho, Jung-Il, Nguyen, Cong Danh, Moon, Sunok, Park, Jeong Eun, Park, Hye Rang, Huh, Jin Hoe, Jung, Ki-Hong, Guiderdoni, Emmanuel, Jeon, Jong-Seong

المساهمون: Kyung Hee University (KHU), Seoul National University Seoul (SNU), Amélioration génétique et adaptation des plantes méditerranéennes et tropicales (UMR AGAP), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Département Systèmes Biologiques (Cirad-BIOS), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad), Next Generation BioGreen 21 Program of the Rural Development Administration of Korea (PJ013172012019), Mid-Career Researcher Program of the National Research Foundation of Korea (NRF-2017R1A2B4009687)

المصدر: ISSN: 0022-0957.

مصطلحات موضوعية: There is little known about the function of rice hexokinases (HXKs) in planta. We characterized hxk5-1, a Tos17 mutant of OsHXK5 that is up-regulated in maturing pollen, a stage when starch accumulates. Progeny analysis of self-pollinated heterozygotes of hxk5-1 and reciprocal crosses between the wild-type and heterozygotes revealed that loss of HXK5 causes male sterility. Homozygous hxk5-1, produced via anther culture, and additional homozygous hxk5-2, hxk5-3 and hxk5-4 lines created by CRISPR/Cas9 confirmed the male-sterile phenotype. In vitro pollen germination ability and in vivo pollen tube growth rate were significantly reduced in the hxk5 mutant pollen. Biochemical analysis of anthers with the mutant pollen revealed significantly reduced hexokinase activity and starch content, although they were sufficient to produce some viable seed. However, the mutant pollen was unable to compete successfully against wild-type pollen. Expression of the catalytically inactive OsHXK5-G113D did not rescue the hxk5 male-sterile phenotype, indicating that its catalytic function was responsible for pollen fertility, rather than its role in sugar sensing and signaling. Our results demonstrate that OsHXK5 contributes to a large portion of the hexokinase activity necessary for the starch utilization pathway during pollen germination and tube growth, as well as for starch biosynthesis during pollen maturation, [SDV.BV]Life Sciences [q-bio]/Vegetal Biology

Relation: info:eu-repo/semantics/altIdentifier/pmid/31671177; hal-03128415; https://hal.inrae.fr/hal-03128415; PUBMED: 31671177; WOS: 000524911100012

الاتاحة: https://hal.inrae.fr/hal-03128415
https://doi.org/10.1093/jxb/erz436