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المؤلفون: Amit Singh, Ballachanda N. Devaiah, Dinah S. Singer, Jocelyn D. Weissman, Ross C. Larue, Peter Schuck
المصدر: The Journal of Biological Chemistry
مصطلحات موضوعية: ET, extra terminal domain, BD1 and BD2, bromodomains 1 and 2, Amino Acid Motifs, RNA polymerase II, Biochemistry, Mice, DDM, n-dodecyl β-D-maltoside, CTM, C-terminal motif, BET, bromodomain and extra terminal, P-TEFb, biology, Chemistry, Nuclear Proteins, TAF7, BID, basic residue-rich interaction domain, Cell biology, A and B, conserved BET motifs, BRD4, RNA Polymerase II, Casein kinase 2, PTEFb, positive transcription elongation factor b, Research Article, SEED, Ser/Glu/Asp-rich region, SEC, size-exclusion chromatography, extended dimer, kinase, CTD, RNA polymerase II carboxy terminal domain, Protein Domains, TCEP, Tris(2-carboxyethyl) phosphine hydrochloride, BRD4, bromodomain protein 4, Animals, Kinase activity, Protein Structure, Quaternary, Km, Michaelis–Menten constant, Molecular Biology, Vmax, maximal rate, TATA-Binding Protein Associated Factors, AUC, analytical ultracentrifugation, Cell Biology, CTD, NPS and CPS, N and C-terminal phosphorylation sites respectively, Bromodomain, CK2, casein kinase 2, Protein kinase domain, biology.protein, MLVIN, murine leukemia virus integrase, Cyclin-dependent kinase 9, Transcription Factor TFIID, Protein Multimerization, Protein Kinases, Transcription Factors
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المؤلفون: Ramberger, Evelyn, Suarez-Artiles, Lorena, Perez-Hernandez, Daniel, Haji, Mohamed, Popp, Oliver, Reimer, Ulf, Leutz, Achim, Dittmar, Gunnar, Mertins, Philipp
المصدر: Molecular & Cellular Proteomics : MCP
مصطلحات موضوعية: Proteomics, FDR, false discovery rate, point mutations, Amino Acid Motifs, PTMs, post-translational modifications, PPIs, protein–protein interactions, IDRs, intrinsically disordered regions, SLiMs, short linear motifs, post-translational modifications, Humans, Point Mutation, AGC, automated gain control, Protein Interaction Domains and Motifs, TCEP, Tris(2-carboxyethyl)phosphine hydrochloride, ABC, ammonium bicarbonate, MBR, match between run, Technological Innovation and Resources, LFQ, label-free quantification, short linear motifs, CAA, chloroacetamide, MoRFs, molecular recognition features, EGFR, epidermal growth factor receptor, peptide interaction screen, CRs, conserved regions, PRISMA, protein interaction screen on a peptide matrix, IT, injection time, Peptides, Protein Processing, Post-Translational, SH2, Src homology 2, HeLa Cells, Protein Binding
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المؤلفون: Suresh, Kumar, Mathivanan, Chinnaraj, William, Planer, Xiaobing, Zuo, Paolo, Macor, Francesco, Tedesco, Nicola, Pozzi
المصدر: The Journal of Biological Chemistry
مصطلحات موضوعية: structure–function, SEC, size-exclusion chromatography, β2GPI, β2-glycoprotein I, SPR, surface plasmon resonance, Crystallography, X-Ray, POPC:POPS, phosphatidylcholine: phosphatidylserine, CCP, complement control protein, Cell Line, vWF, von Willebrand Factor, lipid–protein interaction, GpIbα, glycoprotein Ib alpha, Allosteric Regulation, Protein Domains, aPL, antiphospholipid, Humans, oxidative stress, coagulation factor, thrombosis, complement system, Autoantibodies, POPC:CL, phosphatidylcholine: cardiolipin, autoimmunity, SAXS, small-angle X-ray scattering, TCEP, tris(2-carboxyethyl)phosphine hydrochloride, Antiphospholipid Syndrome, protein disulfide isomerase, LA, lupus anticoagulant, Recombinant Proteins, allosteric regulation, APS, antiphospholipid syndrome, beta 2-Glycoprotein I, aCL, anticardiolipin, Antibodies, Antiphospholipid, LUV, large unilamellar vesicle, MPB, 3-(N-maleimido-propionyl) biocytin, Oxidation-Reduction, antiphospholipid syndrome, Research Article, disulfide
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المؤلفون: Michael J. Davies, Shuwen Jiang, Per Hägglund, Luke Carroll, Michele Mariotti
المصدر: Jiang, S, Carroll, L, Mariotti, M, Hagglund, P & Davies, M J 2021, ' Formation of protein cross-links by singlet oxygen-mediated disulfide oxidation ', Redox Biology, vol. 41, 101874 . https://doi.org/10.1016/j.redox.2021.101874
Redox Biology, Vol 41, Iss, Pp 101874-(2021)
Redox Biologyمصطلحات موضوعية: 0301 basic medicine, Medicine (General), Clinical Biochemistry, Protein aggregation, Biochemistry, chemistry.chemical_compound, 0302 clinical medicine, Photo-oxidation, Disulfides, Biology (General), PDB, protein data bank, Thiol-disulfide exchange, Thiosulfinate, MOLECULAR-OXYGEN, FA, formic acid, chemistry.chemical_classification, Singlet oxygen, PBST, phosphate-buffered saline containing Tween 20, TCEP, tris(2-carboxyethyl)phosphine hydrochloride, Glutathione, INDUCED ELECTROSTATIC STABILIZATION, BONDS, GAPDH, glyceraldehyde-3-phosphate dehydrogenase, IAM, iodoacetamide, DTT, DL-Dithiothreitol, INACTIVATION, Post-translational modification, Lysozyme, Oxidation-Reduction, Research Paper, Stereochemistry, QH301-705.5, CRP, recombinant human C-reactive protein, Redox, Enzyme catalysis, B2M, β-2 microglobulin, MECHANISMS, TFA, trifluoroacetic acid, 03 medical and health sciences, Disulfide, LYSO, lysozyme, R5-920, SULFENIC ACIDS, TYROSINE, BETA(2)-MICROGLOBULIN, aLA, α-Lactalbumin, Humans, GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE, Organic Chemistry, Proteins, ACN, acetonitrile, 030104 developmental biology, Enzyme, chemistry, NEM, N-ethylmaleimide, Protein cross-links, PHOTOOXIDATION, RB, Rose Bengal, 030217 neurology & neurosurgery
وصف الملف: application/pdf
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المؤلفون: Dayanand Kalyani, Markus M. Keskitalo, Henrik Aspeborg, Tom Reichenbach, Julian Conrad, Christina Divne
المصدر: Journal of Structural Biology: X, Vol 5, Iss, Pp 100048-(2021)
Journal of Structural Biology: Xمصطلحات موضوعية: Mannosidase, Reticulitermes, Structural Biology, TCEP, tris (2-carboxyethyl) phosphine hydrochloride, Glycoside hydrolase, Fuc, fucopyranoside, Xyl, xylopyranoside, Biology (General), HPAEC-PAD, High Performance Anion Exchange Chromatography and Pulsed Amperometric Detection, Gal, galactopyranoside, SDS-PAGE, sodium dodecyl sulfate–polyacrylamide gel electrophoresis, Mannan, biology, Termite hindgut, Chemistry, food and beverages, Hindgut, IPTG, β-D-1-thiogalactopyranoside, MWCO, molecular weight cut-off, Glycosyl hydrolase family 5, CAZy, Carbohydrate-Active enZymes database, Biochemistry, Bacteroides salyersiae, SEC, size-exclusion chromatography, cryo-EM, electron cryo-microscopy, 4-mannosidase, QH301-705.5, Article, Electron cryo-microscopy, Glc, glucopyranoside, MOS, mannooligosaccharides, Reticulitermes flavipes, Verrucomicrobia, GH, glycoside hydrolase, ESI-MS, electrospray ionization mass spectrometry, ComputingMethodologies_COMPUTERGRAPHICS, EDTA, ethylenediaminetetraacetic acid, CMC, carboxymethyl cellulose, Glycoside hydrolase family 5, Crystal structure, Opitutaceae, HEPES, 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid, Op5Man5, exo-β-1,4-mannosidase from Opitutaceae bacterium strain TAV5, DP, degree of polymerization, TLC, thin-layer chromatography, biology.organism_classification, GlcNAc, N-acetyl glucosamine, LBG, locust bean gum, Exo-β-1, pNP, p-nitrophenyl, Man, mannopyranoside
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المؤلفون: Xiao Xu, Chuan-Qi Zhong, Guohong Gan, Jinling Wang, Xiu-Fang Zhang, Xi Chen
المصدر: Molecular & Cellular Proteomics : MCP
مصطلحات موضوعية: Proteomics, Swath ms, BAEE, Nα-benzoyl-l-arginine ethyl ester hydrochloride, FDR, false discovery rate, Electrospray ionization, SCASP, SDS–CD-assisted sample preparation, SP3, single-pot and solid-phase–enhanced sample preparation, FFPE, formalin-fixed and paraffin-embedded, SDC, Biochemistry, Cell Line, Analytical Chemistry, TFA, trifluoroacetic acid, 03 medical and health sciences, SDB-RPS, poly(styrenedivinylbenzene)-reversed phase sulfonate, FASP, filter-aided sample preparation, Animals, Humans, iRT, independent retention time, Trypsin, Sample preparation, SWATH-MS, Sequential Window Acquisition of All Theoretical Mass Spectra, SDS, TCEP, Tris(2-carboxyethyl)phosphine hydrochloride, Molecular Biology, Sodium Deoxycholate, FA, formic acid, 030304 developmental biology, Cyclodextrins, CAA, 2-chloroacetamide, 0303 health sciences, Chromatography, sample preparation, Chemistry, 030302 biochemistry & molecular biology, Technological Innovation and Resources, Sodium Dodecyl Sulfate, ACN, acetonitrile, PASEF, parallel accumulation—serial fragmentation, Mice, Inbred C57BL, CD, cyclodextrin, iST, in-StageTip, DDA, data-dependent acquisition, SDC, sodium deoxycholate
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المؤلفون: Shigeki Ono, Noritoshi Kitamoto, Yoji Kato, Anthony J. Kettle
المصدر: Redox Biology
Redox Biology, Vol 2, Iss C, Pp 983-990 (2014)مصطلحات موضوعية: Tryptamine, TTBS, Tris-buffered saline containing 0.05% Tween-20, NBT, nitroblue tetrazolium, GAPDH, glyceraldehyde 3-phosphate dehydrogenase, Clinical Biochemistry, HOCl, hypochlorous acid, XOD, xanthine oxidase, Biochemistry, DMEM, Dulbecco’s modified Eagle’s medium, chemistry.chemical_compound, 5OH-Trp, 5-hydroxytryptophan, Cytoskeleton, lcsh:QH301-705.5, Neurons, lcsh:R5-920, biology, Chemistry, HRP, horseradish peroxidase, 5-Hydroxytryptamine, ELISA, enzyme-linked immunosorbent assay, Tryptamines, Blot, AP, alkaline phosphatase, PMNs, polymorphonuclear leukocytes, Myeloperoxidase, TMB, 3,3′,5,5′-tetramethylbenzidine reagent, Quinone, lcsh:Medicine (General), Adduct, Research Paper, SDS, sodium dodecyl sulfate, PBS, phosphate-buffered saline, Nerve Tissue Proteins, O.D., optical density, TD, tryptamine-4,5-dione, AD, Alzheimer’s disease, Tryptamine 4,5-dione, In vivo, Cell Line, Tumor, Humans, Indolequinones, Antibody, Organic Chemistry, NAC, N-acetyl-l-cysteine, 5HIAA, 5-hydroxyindoleacetic acid, In vitro, TCEP, Tris[2-carboxyethyl] phosphine hydrochloride, Cytoskeletal Proteins, Cytosol, lcsh:Biology (General), DTT, dithiothreitol, Neuronal cells, NEM, N-ethylmaleimide, biology.protein, KLH, keyhole limpet hemocyanin, BSA, bovine serum albumin, Serotonin, Protein Processing, Post-Translational, TPBS, PBS containing 0.05% Tween 20
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المؤلفون: Juan José, Sánchez-Romero, Luis F, Olguin
المصدر: Biochemistry and Biophysics Reports
مصطلحات موضوعية: DLS, dynamic light scattering, ITC, isothermal titration calorimetry, anSME, anaerobic sulfatase maturing enzyme, MALDI-TOF, matrix assisted laser desorption ionization time-of-flight, pNPS, p-nitrophenyl sulfate, Choline-O-sulfatase, DTNB, 5,5′-Dithiobis(2-nitrobenzoic acid), COS, E. meliloti choline-O-sulfatase, Type I sulfatase, FGly, α-formylglycine, Catalytic efficiency, UPLC-ESI-Q-TOF-MS, Ultra-performance liquid chromatography-electrospray ionization-quadrupole time-of-flight-mass spectrometry, Formylglycine post-translational modification, bacteria, DTT, DL-Dithiothreitol, Choline-O-sulfate, FGE, α-formylglycine-generating enzyme, MUS, 4-methylumbelliferyl sulfate, TCEP, Tris(2-carboxyethyl)phosphine hydrochloride, Research Article