المؤلفون: Pascal Hoffmann (11320516), Nadine Schnepel (11320519), Marion Langeheine (2633689), Katrin Künnemann (11320522), Guntram A. Grassl (8777744), Ralph Brehm (494774), Bettina Seeger (848609), Gemma Mazzuoli-Weber (11320525), Gerhard Breves (511939)

مصطلحات موضوعية: Microbiology, Cell Biology, Genetics, Molecular Biology, Physiology, Ecology, Developmental Biology, Infectious Diseases, Environmental Sciences not elsewhere classified, Biological Sciences not elsewhere classified, Chemical Sciences not elsewhere classified, Information Systems not elsewhere classified, organoid-based 2 D monolayers, pig intestine Gastrointestinal, Intestinal organoid-based 2 D monol., 3 R principles, cholera toxin, 3 D Organoids, 3 R principle, epithelial chloride secretion, pathophysiological, 2 D epithelial monolayers, species-specific, model

Relation: https://figshare.com/articles/journal_contribution/Differentiation_medium_composition_/16413868

الاتاحة: https://doi.org/10.1371/journal.pone.0256143.s004

المؤلفون: Pascal Hoffmann (11320516), Nadine Schnepel (11320519), Marion Langeheine (2633689), Katrin Künnemann (11320522), Guntram A. Grassl (8777744), Ralph Brehm (494774), Bettina Seeger (848609), Gemma Mazzuoli-Weber (11320525), Gerhard Breves (511939)

مصطلحات موضوعية: Microbiology, Cell Biology, Genetics, Molecular Biology, Physiology, Ecology, Developmental Biology, Infectious Diseases, Environmental Sciences not elsewhere classified, Biological Sciences not elsewhere classified, Chemical Sciences not elsewhere classified, Information Systems not elsewhere classified, organoid-based 2 D monolayers, pig intestine Gastrointestinal, Intestinal organoid-based 2 D monol., 3 R principles, cholera toxin, 3 D Organoids, 3 R principle, epithelial chloride secretion, pathophysiological, 2 D epithelial monolayers, species-specific, model

Relation: https://figshare.com/articles/journal_contribution/Monolayer_medium_composition_/16413865

الاتاحة: https://doi.org/10.1371/journal.pone.0256143.s003

المؤلفون: Pascal Hoffmann (11320516), Nadine Schnepel (11320519), Marion Langeheine (2633689), Katrin Künnemann (11320522), Guntram A. Grassl (8777744), Ralph Brehm (494774), Bettina Seeger (848609), Gemma Mazzuoli-Weber (11320525), Gerhard Breves (511939)

مصطلحات موضوعية: Microbiology, Cell Biology, Genetics, Molecular Biology, Physiology, Ecology, Developmental Biology, Infectious Diseases, Environmental Sciences not elsewhere classified, Biological Sciences not elsewhere classified, Chemical Sciences not elsewhere classified, Information Systems not elsewhere classified, organoid-based 2 D monolayers, pig intestine Gastrointestinal, Intestinal organoid-based 2 D monol., 3 R principles, cholera toxin, 3 D Organoids, 3 R principle, epithelial chloride secretion, pathophysiological, 2 D epithelial monolayers, species-specific, model

Relation: https://figshare.com/articles/journal_contribution/Primers_used_for_gene_expression_quantification_/16413862

الاتاحة: https://doi.org/10.1371/journal.pone.0256143.s002

المؤلفون: Pascal Hoffmann (11320516), Nadine Schnepel (11320519), Marion Langeheine (2633689), Katrin Künnemann (11320522), Guntram A. Grassl (8777744), Ralph Brehm (494774), Bettina Seeger (848609), Gemma Mazzuoli-Weber (11320525), Gerhard Breves (511939)

مصطلحات موضوعية: Microbiology, Cell Biology, Genetics, Molecular Biology, Physiology, Ecology, Developmental Biology, Infectious Diseases, Environmental Sciences not elsewhere classified, Biological Sciences not elsewhere classified, Chemical Sciences not elsewhere classified, Information Systems not elsewhere classified, organoid-based 2 D monolayers, pig intestine Gastrointestinal, Intestinal organoid-based 2 D monol., 3 R principles, cholera toxin, 3 D Organoids, 3 R principle, epithelial chloride secretion, pathophysiological, 2 D epithelial monolayers, species-specific, model

Relation: https://figshare.com/articles/journal_contribution/Culture_medium_composition_/16413859

الاتاحة: https://doi.org/10.1371/journal.pone.0256143.s001

المؤلفون: Pascal Hoffmann (11320516), Nadine Schnepel (11320519), Marion Langeheine (2633689), Katrin Künnemann (11320522), Guntram A. Grassl (8777744), Ralph Brehm (494774), Bettina Seeger (848609), Gemma Mazzuoli-Weber (11320525), Gerhard Breves (511939)

مصطلحات موضوعية: Microbiology, Cell Biology, Genetics, Molecular Biology, Physiology, Ecology, Developmental Biology, Infectious Diseases, Environmental Sciences not elsewhere classified, Biological Sciences not elsewhere classified, Chemical Sciences not elsewhere classified, Information Systems not elsewhere classified, organoid-based 2 D monolayers, pig intestine Gastrointestinal, Intestinal organoid-based 2 D monol., 3 R principles, cholera toxin, 3 D Organoids, 3 R principle, epithelial chloride secretion, pathophysiological, 2 D epithelial monolayers, species-specific, model

Relation: https://figshare.com/articles/journal_contribution/Composition_of_the_buffer_solutions_used_for_Ussing_chamber_experiments_all_chemicals_were_obtained_from_Sigma-Aldrich_Darmstadt_Germany_and_diluted_in_i_aqua_destillata_i_/16413871

الاتاحة: https://doi.org/10.1371/journal.pone.0256143.s005

المؤلفون: Pascal Hoffmann (11320516), Marion Burmester (4836936), Marion Langeheine (2633689), Ralph Brehm (494774), Michael T. Empl (1510813), Bettina Seeger (848609), Gerhard Breves (511939)

مصطلحات موضوعية: Biophysics, Biochemistry, Medicine, Microbiology, Cell Biology, Genetics, Molecular Biology, Physiology, Biotechnology, Developmental Biology, Cancer, Inorganic Chemistry, Infectious Diseases, Biological Sciences not elsewhere classified, Chemical Sciences not elsewhere classified, structural level combined, measuring protein expression, human intestinal epithelium, high electrical resistance, characterizing pathophysiological mechanisms, induced secretory diarrhea, induced disease mechanisms, physiological intestinal properties, apical chloride secretion, studying physiological properties, typical cholera toxin, functional epithelial barrier, physiological properties, chloride secretion, induced effects

Relation: https://figshare.com/articles/journal_contribution/Raw_images_of_Western_Blot_membranes_of_pSGLT1_SGLT1_villin_total_protein_content_PepT1_and_CFTR_/16765161

الاتاحة: https://doi.org/10.1371/journal.pone.0257824.s002

المؤلفون: Pascal Hoffmann (11320516), Nadine Schnepel (11320519), Marion Langeheine (2633689), Katrin Künnemann (11320522), Guntram A. Grassl (8777744), Ralph Brehm (494774), Bettina Seeger (848609), Gemma Mazzuoli-Weber (11320525), Gerhard Breves (511939)

مصطلحات موضوعية: Microbiology, Cell Biology, Genetics, Molecular Biology, Physiology, Ecology, Developmental Biology, Infectious Diseases, Environmental Sciences not elsewhere classified, Biological Sciences not elsewhere classified, Chemical Sciences not elsewhere classified, Information Systems not elsewhere classified, organoid-based 2 D monolayers, pig intestine Gastrointestinal, Intestinal organoid-based 2 D monol., 3 R principles, cholera toxin, 3 D Organoids, 3 R principle, epithelial chloride secretion, pathophysiological, 2 D epithelial monolayers, species-specific, model

Relation: https://figshare.com/articles/figure/Cellular_arrangement_cross_sections_of_jejunum_organoids_grown_in_2D_on_Snapwells_sup_sup_for_18_days_/16413883

الاتاحة: https://doi.org/10.1371/journal.pone.0256143.g004

المؤلفون: Pascal Hoffmann (11320516), Nadine Schnepel (11320519), Marion Langeheine (2633689), Katrin Künnemann (11320522), Guntram A. Grassl (8777744), Ralph Brehm (494774), Bettina Seeger (848609), Gemma Mazzuoli-Weber (11320525), Gerhard Breves (511939)

مصطلحات موضوعية: Microbiology, Cell Biology, Genetics, Molecular Biology, Physiology, Ecology, Developmental Biology, Infectious Diseases, Environmental Sciences not elsewhere classified, Biological Sciences not elsewhere classified, Chemical Sciences not elsewhere classified, Information Systems not elsewhere classified, organoid-based 2 D monolayers, pig intestine Gastrointestinal, Intestinal organoid-based 2 D monol., 3 R principles, cholera toxin, 3 D Organoids, 3 R principle, epithelial chloride secretion, pathophysiological, 2 D epithelial monolayers, species-specific, model

Relation: https://figshare.com/articles/figure/Cellular_arrangement_cross_sections_of_jejunum_organoids_grown_in_2D_on_Snapwells_sup_sup_for_18_days_/16413892

الاتاحة: https://doi.org/10.1371/journal.pone.0256143.g005

المؤلفون: Pascal Hoffmann (11320516), Nadine Schnepel (11320519), Marion Langeheine (2633689), Katrin Künnemann (11320522), Guntram A. Grassl (8777744), Ralph Brehm (494774), Bettina Seeger (848609), Gemma Mazzuoli-Weber (11320525), Gerhard Breves (511939)

مصطلحات موضوعية: Microbiology, Cell Biology, Genetics, Molecular Biology, Physiology, Ecology, Developmental Biology, Infectious Diseases, Environmental Sciences not elsewhere classified, Biological Sciences not elsewhere classified, Chemical Sciences not elsewhere classified, Information Systems not elsewhere classified, organoid-based 2 D monolayers, pig intestine Gastrointestinal, Intestinal organoid-based 2 D monol., 3 R principles, cholera toxin, 3 D Organoids, 3 R principle, epithelial chloride secretion, pathophysiological, 2 D epithelial monolayers, species-specific, model

Relation: https://figshare.com/articles/figure/Changes_of_transepithelial_electrical_resistance_TEER_values_over_the_18_day_culture_period_on_Snapwells_sup_sup_of_organoid-based_2D_monolayers_as_a_function_of_time_/16413880

الاتاحة: https://doi.org/10.1371/journal.pone.0256143.g003

المؤلفون: Pascal Hoffmann (11320516), Nadine Schnepel (11320519), Marion Langeheine (2633689), Katrin Künnemann (11320522), Guntram A. Grassl (8777744), Ralph Brehm (494774), Bettina Seeger (848609), Gemma Mazzuoli-Weber (11320525), Gerhard Breves (511939)

مصطلحات موضوعية: Microbiology, Cell Biology, Genetics, Molecular Biology, Physiology, Ecology, Developmental Biology, Infectious Diseases, Environmental Sciences not elsewhere classified, Biological Sciences not elsewhere classified, Chemical Sciences not elsewhere classified, Information Systems not elsewhere classified, organoid-based 2 D monolayers, pig intestine Gastrointestinal, Intestinal organoid-based 2 D monol., 3 R principles, cholera toxin, 3 D Organoids, 3 R principle, epithelial chloride secretion, pathophysiological, 2 D epithelial monolayers, species-specific, model

Relation: https://figshare.com/articles/figure/Gene_expression_of_sodium_glucose_cotransporter_1_i_SGLT1_i_cystic_fibrosis_transmembrane_conductance_regulator_i_CFTR_i_and_mucin_2_i_MUC2_i_in_intestinal_organoids_of_the_porcine_jejunum_cultivated_for_seven_days_in_comparison_to_native_t/16413877

الاتاحة: https://doi.org/10.1371/journal.pone.0256143.g002

المؤلفون: Pascal Hoffmann (11320516), Nadine Schnepel (11320519), Marion Langeheine (2633689), Katrin Künnemann (11320522), Guntram A. Grassl (8777744), Ralph Brehm (494774), Bettina Seeger (848609), Gemma Mazzuoli-Weber (11320525), Gerhard Breves (511939)

مصطلحات موضوعية: Microbiology, Cell Biology, Genetics, Molecular Biology, Physiology, Ecology, Developmental Biology, Infectious Diseases, Environmental Sciences not elsewhere classified, Biological Sciences not elsewhere classified, Chemical Sciences not elsewhere classified, Information Systems not elsewhere classified, organoid-based 2 D monolayers, pig intestine Gastrointestinal, Intestinal organoid-based 2 D monol., 3 R principles, cholera toxin, 3 D Organoids, 3 R principle, epithelial chloride secretion, pathophysiological, 2 D epithelial monolayers, species-specific, model

Relation: https://figshare.com/articles/figure/Intestinal_porcine_crypts_isolated_from_the_jejunum_D0_form_3D-organoids_D7_after_a_cultivation_period_of_seven_days_/16413874

الاتاحة: https://doi.org/10.1371/journal.pone.0256143.g001

المؤلفون: Pascal Hoffmann (11320516), Nadine Schnepel (11320519), Marion Langeheine (2633689), Katrin Künnemann (11320522), Guntram A. Grassl (8777744), Ralph Brehm (494774), Bettina Seeger (848609), Gemma Mazzuoli-Weber (11320525), Gerhard Breves (511939)

مصطلحات موضوعية: Microbiology, Cell Biology, Genetics, Molecular Biology, Physiology, Ecology, Developmental Biology, Infectious Diseases, Environmental Sciences not elsewhere classified, Biological Sciences not elsewhere classified, Chemical Sciences not elsewhere classified, Information Systems not elsewhere classified, organoid-based 2 D monolayers, pig intestine Gastrointestinal, Intestinal organoid-based 2 D monol., 3 R principles, cholera toxin, 3 D Organoids, 3 R principle, epithelial chloride secretion, pathophysiological, 2 D epithelial monolayers, species-specific, model

Relation: https://figshare.com/articles/figure/Cellular_arrangement_cross_sections_of_jejunum_organoids_grown_in_2D_on_Snapwells_sup_sup_for_18_days_/16413898

الاتاحة: https://doi.org/10.1371/journal.pone.0256143.g006

المؤلفون: Pascal Hoffmann (11320516), Nadine Schnepel (11320519), Marion Langeheine (2633689), Katrin Künnemann (11320522), Guntram A. Grassl (8777744), Ralph Brehm (494774), Bettina Seeger (848609), Gemma Mazzuoli-Weber (11320525), Gerhard Breves (511939)

مصطلحات موضوعية: Microbiology, Cell Biology, Genetics, Molecular Biology, Physiology, Ecology, Developmental Biology, Infectious Diseases, Environmental Sciences not elsewhere classified, Biological Sciences not elsewhere classified, Chemical Sciences not elsewhere classified, Information Systems not elsewhere classified, organoid-based 2 D monolayers, pig intestine Gastrointestinal, Intestinal organoid-based 2 D monol., 3 R principles, cholera toxin, 3 D Organoids, 3 R principle, epithelial chloride secretion, pathophysiological, 2 D epithelial monolayers, species-specific, model

Relation: https://figshare.com/articles/figure/Immunofluorescence_staining_cross_sections_of_jejunum_organoids_grown_in_2D_on_Snapwells_sup_sup_for_18_days_/16413904

الاتاحة: https://doi.org/10.1371/journal.pone.0256143.g007

المؤلفون: Pascal Hoffmann (11320516), Nadine Schnepel (11320519), Marion Langeheine (2633689), Katrin Künnemann (11320522), Guntram A. Grassl (8777744), Ralph Brehm (494774), Bettina Seeger (848609), Gemma Mazzuoli-Weber (11320525), Gerhard Breves (511939)

مصطلحات موضوعية: Microbiology, Cell Biology, Genetics, Molecular Biology, Physiology, Ecology, Developmental Biology, Infectious Diseases, Environmental Sciences not elsewhere classified, Biological Sciences not elsewhere classified, Chemical Sciences not elsewhere classified, Information Systems not elsewhere classified, organoid-based 2 D monolayers, pig intestine Gastrointestinal, Intestinal organoid-based 2 D monol., 3 R principles, cholera toxin, 3 D Organoids, 3 R principle, epithelial chloride secretion, pathophysiological, 2 D epithelial monolayers, species-specific, model

Relation: https://figshare.com/articles/figure/Basal_and_maximal_I_sub_sc_sub_and_R_sub_t_sub_values_of_organoid-based_2D_monolayers_cultivated_for_18_days_determined_before_and_after_subsequent_addition_of_CTX_forskolin_and_ouabain_/16413916

الاتاحة: https://doi.org/10.1371/journal.pone.0256143.g009

المؤلفون: Pascal Hoffmann (11320516), Nadine Schnepel (11320519), Marion Langeheine (2633689), Katrin Künnemann (11320522), Guntram A. Grassl (8777744), Ralph Brehm (494774), Bettina Seeger (848609), Gemma Mazzuoli-Weber (11320525), Gerhard Breves (511939)

مصطلحات موضوعية: Microbiology, Cell Biology, Genetics, Molecular Biology, Physiology, Ecology, Developmental Biology, Infectious Diseases, Environmental Sciences not elsewhere classified, Biological Sciences not elsewhere classified, Chemical Sciences not elsewhere classified, Information Systems not elsewhere classified, organoid-based 2 D monolayers, pig intestine Gastrointestinal, Intestinal organoid-based 2 D monol., 3 R principles, cholera toxin, 3 D Organoids, 3 R principle, epithelial chloride secretion, pathophysiological, 2 D epithelial monolayers, species-specific, model

Relation: https://figshare.com/articles/figure/Basal_and_maximal_I_sub_sc_sub_and_R_sub_t_sub_values_of_organoid-based_2D_monolayers_cultivated_for_18_days_determined_before_and_after_subsequent_addition_of_glucose_carbachol_and_forskolin_/16413910

الاتاحة: https://doi.org/10.1371/journal.pone.0256143.g008

المؤلفون: Pascal Hoffmann (11320516), Marion Burmester (4836936), Marion Langeheine (2633689), Ralph Brehm (494774), Michael T. Empl (1510813), Bettina Seeger (848609), Gerhard Breves (511939)

مصطلحات موضوعية: Biophysics, Biochemistry, Medicine, Microbiology, Cell Biology, Genetics, Molecular Biology, Physiology, Biotechnology, Developmental Biology, Cancer, Inorganic Chemistry, Infectious Diseases, Biological Sciences not elsewhere classified, Chemical Sciences not elsewhere classified, structural level combined, measuring protein expression, human intestinal epithelium, high electrical resistance, characterizing pathophysiological mechanisms, induced secretory diarrhea, induced disease mechanisms, physiological intestinal properties, apical chloride secretion, studying physiological properties, typical cholera toxin, functional epithelial barrier, physiological properties, chloride secretion, induced effects

Relation: https://figshare.com/articles/dataset/S1_File_-/16765158

الاتاحة: https://doi.org/10.1371/journal.pone.0257824.s001

المؤلفون: Pascal Hoffmann (11320516), Marion Burmester (4836936), Marion Langeheine (2633689), Ralph Brehm (494774), Michael T. Empl (1510813), Bettina Seeger (848609), Gerhard Breves (511939)

مصطلحات موضوعية: Biophysics, Biochemistry, Medicine, Microbiology, Cell Biology, Genetics, Molecular Biology, Physiology, Biotechnology, Developmental Biology, Cancer, Inorganic Chemistry, Infectious Diseases, Biological Sciences not elsewhere classified, Chemical Sciences not elsewhere classified, structural level combined, measuring protein expression, human intestinal epithelium, high electrical resistance, characterizing pathophysiological mechanisms, induced secretory diarrhea, induced disease mechanisms, physiological intestinal properties, apical chloride secretion, studying physiological properties, typical cholera toxin, functional epithelial barrier, physiological properties, chloride secretion, induced effects

Relation: https://figshare.com/articles/figure/Changes_of_TEER_values_as_a_function_of_time_of_Caco-2_HT29-MTX_and_co-cultured_Caco-2_and_HT29-MTX_cells_incubated_for_32_days_on_Snapwells_sup_sup_/16765164

الاتاحة: https://doi.org/10.1371/journal.pone.0257824.g001

المؤلفون: Pascal Hoffmann (11320516), Marion Burmester (4836936), Marion Langeheine (2633689), Ralph Brehm (494774), Michael T. Empl (1510813), Bettina Seeger (848609), Gerhard Breves (511939)

مصطلحات موضوعية: Biophysics, Biochemistry, Medicine, Microbiology, Cell Biology, Genetics, Molecular Biology, Physiology, Biotechnology, Developmental Biology, Cancer, Inorganic Chemistry, Infectious Diseases, Biological Sciences not elsewhere classified, Chemical Sciences not elsewhere classified, structural level combined, measuring protein expression, human intestinal epithelium, high electrical resistance, characterizing pathophysiological mechanisms, induced secretory diarrhea, induced disease mechanisms, physiological intestinal properties, apical chloride secretion, studying physiological properties, typical cholera toxin, functional epithelial barrier, physiological properties, chloride secretion, induced effects

Relation: https://figshare.com/articles/figure/Immunohistochemical_staining_including_isotype_controls_of_ZO-1_cross-section_in_Caco-2_HT29-MTX_as_well_as_co-cultured_Caco-2_and_HT29-MTX_cells_cultivated_for_7_D7_and_28_days_D28_/16765167

الاتاحة: https://doi.org/10.1371/journal.pone.0257824.g002

المؤلفون: Pascal Hoffmann (11320516), Marion Burmester (4836936), Marion Langeheine (2633689), Ralph Brehm (494774), Michael T. Empl (1510813), Bettina Seeger (848609), Gerhard Breves (511939)

مصطلحات موضوعية: Biophysics, Biochemistry, Medicine, Microbiology, Cell Biology, Genetics, Molecular Biology, Physiology, Biotechnology, Developmental Biology, Cancer, Inorganic Chemistry, Infectious Diseases, Biological Sciences not elsewhere classified, Chemical Sciences not elsewhere classified, structural level combined, measuring protein expression, human intestinal epithelium, high electrical resistance, characterizing pathophysiological mechanisms, induced secretory diarrhea, induced disease mechanisms, physiological intestinal properties, apical chloride secretion, studying physiological properties, typical cholera toxin, functional epithelial barrier, physiological properties, chloride secretion, induced effects

Relation: https://figshare.com/articles/figure/Immunofluorescence_staining_top_view_of_ZO-1_in_Caco-2_HT29-MTX_and_co-cultured_Caco-2_and_HT29-MTX_cells_cultivated_for_7_D7_and_28_days_D28_/16765170

الاتاحة: https://doi.org/10.1371/journal.pone.0257824.g003

المؤلفون: Pascal Hoffmann (11320516), Marion Burmester (4836936), Marion Langeheine (2633689), Ralph Brehm (494774), Michael T. Empl (1510813), Bettina Seeger (848609), Gerhard Breves (511939)

مصطلحات موضوعية: Biophysics, Biochemistry, Medicine, Microbiology, Cell Biology, Genetics, Molecular Biology, Physiology, Biotechnology, Developmental Biology, Cancer, Inorganic Chemistry, Infectious Diseases, Biological Sciences not elsewhere classified, Chemical Sciences not elsewhere classified, structural level combined, measuring protein expression, human intestinal epithelium, high electrical resistance, characterizing pathophysiological mechanisms, induced secretory diarrhea, induced disease mechanisms, physiological intestinal properties, apical chloride secretion, studying physiological properties, typical cholera toxin, functional epithelial barrier, physiological properties, chloride secretion, induced effects

Relation: https://figshare.com/articles/figure/Cellular_arrangement_cross_sections_of_cells_grown_on_Snapwells_sup_sup_for_28_days_/16765173

الاتاحة: https://doi.org/10.1371/journal.pone.0257824.g004