المؤلفون: Jiajun Chen (1279269), Ding Chen (17069), Qiuming Chen (845173), Wei Xu (28953), Wenli Zhang (146408), Wanmeng Mu (408651)

مصطلحات موضوعية: Biophysics, Biochemistry, Medicine, Microbiology, Genetics, Molecular Biology, Biotechnology, Hematology, Computational Biology, Biological Sciences not elsewhere classified, Chemical Sciences not elsewhere classified, Physical Sciences not elsewhere classified, Information Systems not elsewhere classified, targeted mutagenesis based, ordered recombination mutagenesis, molecular dynamics simulations, increased hydrogen bonds, enhanced overall rigidity, aided targeted mutagenesis, 65 ° c, 60 ° c, 25 ° c, 140 times higher, 10 ° c, optimum temperature (<, thermoclostridium caenicola suffers, opt , combinational mutant var3, ), 2

Relation: https://figshare.com/articles/journal_contribution/Computer-Aided_Targeted_Mutagenesis_of_Thermoclostridium_caenicola_d_Allulose_3_Epimerase_for_Improved_Thermostability/19107983

الاتاحة: https://doi.org/10.1021/acs.jafc.1c07256.s001

المؤلفون: Dawei Ni (8842814), Shuqi Zhang (711493), Onur Kırtel (11535300), Wei Xu (28953), Qiuming Chen (845173), Ebru Toksoy Öner (11535303), Wanmeng Mu (408651)

مصطلحات موضوعية: Biophysics, Biochemistry, Microbiology, Genetics, Biological Sciences not elsewhere classified, Chemical Sciences not elsewhere classified, synergistic promotion effects, multiple sequence alignment, combinational mutation method, 120 times compared, 1 ° c, rational design based, microbial inulin thermostability, construct mutant m4, ), rational engineering, >< sub, variants a446e, surface loop, simulations illustrated, point mutants, optimal ph, molecular dynamics, modeled structure, industrial scale, b <, >- factor, 33 residues

Relation: https://figshare.com/articles/journal_contribution/Improving_the_Thermostability_and_Catalytic_Activity_of_an_Inulosucrase_by_Rational_Engineering_for_the_Biosynthesis_of_Microbial_Inulin/16766640

الاتاحة: https://doi.org/10.1021/acs.jafc.1c04852.s001