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المؤلفون: Thomas A. Bunch, Piyali Guhathakurta, Andrew R. Thompson, Brett A. Colson, David D. Thomas, Victoria C. Lepak, Rhye Samuel Kanassatega, Anna Wilson
المصدر: The Journal of Biological Chemistry
مصطلحات موضوعية: 0301 basic medicine, Time Factors, HCM, hypertrophic cardiomyopathy, AF568, Alexa Fluor 568, library of pharmacologically active compounds (LOPAC), Biosensing Techniques, cardiac myosin-binding protein C (cMyBP-C), Biochemistry, IAEDANS, 5-((((2-iodoacetyl)amino)ethyl)amino)naphtalene-1-sulfonic acid, chemistry.chemical_compound, cMyBP-C, cardiac myosin-binding protein C, high-throughput screening (HTS), DO, donor only, TR-FRET, time-resolved fluorescence energy transfer, Myosin, Fluorescence Resonance Energy Transfer, DA, donor plus acceptor, TMR, tetramethylrhodamine, DWR, direct waveform recording, DCM, dilated cardiomyopathy, AF546, Alexa Fluor 546, SE, standard errors, phosphorylation, FLTPR, fluorescence lifetime plate reader, ITC, isothermal titration calorimetry, P/A, proline/alanine-rich linker between domains C0 and C1, HTS, high-throughput screen, TR-F, time-resolved fluorescence, protein kinase A (PKA), contractile proteins, LOPAC, library of pharmacologically active compounds, Rabbits, DMF, dimethylformamide, actin, Kd, dissociation constant, Protein Binding, Research Article, Sarcomeres, cardiac muscle, site-directed spectroscopy, TCEP, tris(2-carboxyethyl)phosphine, C0-C2, N-terminal fragment of cMyBP-C comprised of C0-P/A-C1-M-C2 domains and linkers, TPA, transient phosphorescence anisotropy, macromolecular substances, Calorimetry, ATA, aurintricarboxylic acid, Filamentous actin, Fluorescence, SD, standard deviations, 03 medical and health sciences, Aurintricarboxylic acid, fluorescence lifetime, Animals, Humans, Binding site, Molecular Biology, Actin, M-ABB, MOPS-actin binding buffer, 030102 biochemistry & molecular biology, M, M-domain, phosphorylatable linker between C1 and C2, Ligand binding assay, Binding protein, Myocardium, Isothermal titration calorimetry, Cell Biology, G-actin, globular actin, ErIA, erythrosine iodoacetamide phosphorescent dye, Actins, High-Throughput Screening Assays, F-actin, filamentous actin, 030104 developmental biology, chemistry, DTT, dithiothreitol, FMAL, fluorescein-5-maleimide, DMSO, dimethylsulphoxide, Bmax, maximum molar binding ratio, Biophysics, BSA, bovine serum albumin, PKA, protein kinase A, Carrier Proteins
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المؤلفون: Robyn T. Rebbeck, Adam W. Avery, David D. Thomas, Anna K. Andrick, Bengt Svensson, Piyali Guhathakurta, Sarah A. Denha, Thomas S. Hays
المصدر: The Journal of Biological Chemistry
مصطلحات موضوعية: 0301 basic medicine, Models, Molecular, Protein Conformation, alpha-Helical, Mutant, Gene Expression, DMSO, dimethyl sulfoxide, Filamin, HTS, high-throughput screening, Biochemistry, Fluorescence Resonance Energy Transfer, Spectrin, time-resolved FRET, FLT, fluorescent lifetime, Cytoskeleton, Drug discovery, Chemistry, Cell biology, Neuroprotective Agents, LOPAC, library of pharmacologically active compounds, HEK293-6E, human embryonic kidney suspension cells expressing the Epstein Barr virus nuclear antigen 1, NPPB, 5-Nitro-2-(3-phenylpropylamino)benzoic acid, fluorescence, swinholide A, Plate reader, Research Article, Protein Binding, actin binding, HEK293-6E cells, CH, calponin homology, F-actin, actin filaments, High-throughput screening, Recombinant Fusion Proteins, Green Fluorescent Proteins, macromolecular substances, FRET, fluorescence resonance energy transfer, Models, Biological, 03 medical and health sciences, fluorescence lifetime, Humans, Spinocerebellar Ataxias, Protein Interaction Domains and Motifs, drug screening, Molecular Biology, Binding Sites, 030102 biochemistry & molecular biology, Reproducibility of Results, Cell Biology, PR, plate reader, SCA5, spinocerebellar ataxia type 5, Actins, High-Throughput Screening Assays, Kinetics, Luminescent Proteins, 030104 developmental biology, Förster resonance energy transfer, HEK293 Cells, Mutation, ABD, actin-binding domain, Marine Toxins, Protein Conformation, beta-Strand
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المؤلفون: Timo K. van den Berg, Elena Kostova, Robin van Bruggen, Pasi Halonen, Thomas R. L. Klei, Boukje M. Beuger, Cor Lieftink, Roderick L. Beijersbergen
المساهمون: Molecular cell biology and Immunology, Graduate School, AII - Amsterdam institute for Infection and Immunity, General Internal Medicine, Landsteiner Laboratory
المصدر: Bioscience Reports
Bioscience Reports, Vol 35, Iss 2, p e00187 (2015)
Kostova, E B, Beuger, B M, Klei, T R L, Halonen, P, Lieftink, C, Beijersbergen, R, van den Berg, T K & van Bruggen, R 2015, ' Identification of signalling cascades involved in red blood cell shrinkage and vesiculation ', Frontiers in Bioscience, vol. 35, no. 2 . https://doi.org/10.1042/BSR20150019
Frontiers in Bioscience, 35(2). Frontiers in Bioscience
Bioscience reports, 35(2). Portland Press Ltd.مصطلحات موضوعية: Erythrocytes, lcsh:Life, lcsh:QR1-502, red blood cell, Cellular Senescence/drug effects, Biochemistry, lcsh:Microbiology, PC, phosphatidylcholine, ERK, extracellular signal-regulated kinase, Cell-Derived Microparticles, Casein Kinase II/metabolism, Jak, Janus kinase, Anaplastic lymphoma kinase, ASK1, Casein Kinase II, Cellular Senescence, CaM, calmodulin, PDGFR, platelet-derived growth factor receptor, AMPK, AMP-activated kinase, Kinase, Ionomycin, RBC, red blood cell, PS, phosphatidylserine, Cell biology, raf Kinases/metabolism, STAT, signal transducer and activator of transcription, Calcium Ionophores, VEGFR, vascular endothelial growth factor receptor, raf Kinases, Casein kinase 2, RTK, receptor tyrosine kinase, SCD, sickle cell disease, Cell aging, PI3K, phosphoinositide 3-kinase, SMase, acid sphingomyelinase, kinase inhibitor, Calcium Ionophores/pharmacology, SAGM, saline-adenine-glucose-mannitol, Biophysics, Biology, ATA, aurintricarboxylic acid, Cell-Derived Microparticles/metabolism, PLC, phospholipase C, bioactive small molecule, PKC, protein kinase C, MEK, mitogen-activated protein kinase kinase, Humans, Protein kinase A, Molecular Biology, Protein kinase B, GPCR, G protein-coupled receptor, BCR-ABL, breakpoint cluster region protein–Abelson murine leukaemia viral oncogene homologue 1, Original Paper, NO, nitric oxide, compound screen, Ionomycin/pharmacology, β-AR, β-adrenergic receptor, Kinase insert domain receptor, Cell Biology, Erythrocytes/cytology, vesiculation, LOPAC, Library of Pharmacologically Active Compounds, nRTK, non-receptor tyrosine kinase, CK2, casein kinase 2, lcsh:QH501-531, Epo, erythropoietin, MAPK, mitogen-activated protein kinase