المؤلفون: Akhila Bettadapur (11768105), Samuel S. Hunter (10099278), Rene L. Suleiman (11768108), Maura C. Ruyechan (11768111), Wesley Huang (6373970), Charles G. Barbieri (11768114), Hannah W. Miller (3716773), Tammie S. Y. Tam (11768117), Matthew L. Settles (9019334), Katherine S. Ralston (11768120)

مصطلحات موضوعية: Medicine, Microbiology, Genetics, Molecular Biology, Developmental Biology, Space Science, Environmental Sciences not elsewhere classified, Biological Sciences not elsewhere classified, Physical Sciences not elsewhere classified, uncovering functional information, uncovered new details, quantify gene fragments, globally important pathogen, div >< p, defining functional domains, novel analysis pipeline, establishing forward genetics, enable forward genetics, based forward genetics, lacked annotated domains, annotated functions consistent, studies dramatically improve, identified slow growth, first rnai screen, entamoeba histolytica <, among genes targeted, sg2 mutants could, forward genetics, histolytica <, targeted genes

Relation: https://figshare.com/articles/figure/Appropriate_sequencing_depth_and_lack_of_bias_during_library_construction_/17095919

الاتاحة: https://doi.org/10.1371/journal.ppat.1010088.s003

المؤلفون: Akhila Bettadapur (11768105), Samuel S. Hunter (10099278), Rene L. Suleiman (11768108), Maura C. Ruyechan (11768111), Wesley Huang (6373970), Charles G. Barbieri (11768114), Hannah W. Miller (3716773), Tammie S. Y. Tam (11768117), Matthew L. Settles (9019334), Katherine S. Ralston (11768120)

مصطلحات موضوعية: Medicine, Microbiology, Genetics, Molecular Biology, Developmental Biology, Space Science, Environmental Sciences not elsewhere classified, Biological Sciences not elsewhere classified, Physical Sciences not elsewhere classified, uncovering functional information, uncovered new details, quantify gene fragments, globally important pathogen, div >< p, defining functional domains, novel analysis pipeline, establishing forward genetics, enable forward genetics, based forward genetics, lacked annotated domains, annotated functions consistent, studies dramatically improve, identified slow growth, first rnai screen, entamoeba histolytica <, among genes targeted, sg2 mutants could, forward genetics, histolytica <, targeted genes

Relation: https://figshare.com/articles/figure/Fragment_Mapping_Analysis_/17095916

الاتاحة: https://doi.org/10.1371/journal.ppat.1010088.s002

المؤلفون: Akhila Bettadapur (11768105), Samuel S. Hunter (10099278), Rene L. Suleiman (11768108), Maura C. Ruyechan (11768111), Wesley Huang (6373970), Charles G. Barbieri (11768114), Hannah W. Miller (3716773), Tammie S. Y. Tam (11768117), Matthew L. Settles (9019334), Katherine S. Ralston (11768120)

مصطلحات موضوعية: Medicine, Microbiology, Genetics, Molecular Biology, Developmental Biology, Space Science, Environmental Sciences not elsewhere classified, Biological Sciences not elsewhere classified, Physical Sciences not elsewhere classified, uncovering functional information, uncovered new details, quantify gene fragments, globally important pathogen, div >< p, defining functional domains, novel analysis pipeline, establishing forward genetics, enable forward genetics, based forward genetics, lacked annotated domains, annotated functions consistent, studies dramatically improve, identified slow growth, first rnai screen, entamoeba histolytica <, among genes targeted, sg2 mutants could, forward genetics, histolytica <, targeted genes

Relation: https://figshare.com/articles/figure/Clonal_transfectants_contain_only_one_library_plasmid_/17095928

الاتاحة: https://doi.org/10.1371/journal.ppat.1010088.s006

المؤلفون: Akhila Bettadapur (11768105), Samuel S. Hunter (10099278), Rene L. Suleiman (11768108), Maura C. Ruyechan (11768111), Wesley Huang (6373970), Charles G. Barbieri (11768114), Hannah W. Miller (3716773), Tammie S. Y. Tam (11768117), Matthew L. Settles (9019334), Katherine S. Ralston (11768120)

مصطلحات موضوعية: Medicine, Microbiology, Genetics, Molecular Biology, Developmental Biology, Space Science, Environmental Sciences not elsewhere classified, Biological Sciences not elsewhere classified, Physical Sciences not elsewhere classified, uncovering functional information, uncovered new details, quantify gene fragments, globally important pathogen, div >< p, defining functional domains, novel analysis pipeline, establishing forward genetics, enable forward genetics, based forward genetics, lacked annotated domains, annotated functions consistent, studies dramatically improve, identified slow growth, first rnai screen, entamoeba histolytica <, among genes targeted, sg2 mutants could, forward genetics, histolytica <, targeted genes

Relation: https://figshare.com/articles/figure/Preparation_of_RNA-free_gDNA_from_i_E_i_i_histolytica_i_/17095910

الاتاحة: https://doi.org/10.1371/journal.ppat.1010088.s001

المؤلفون: Akhila Bettadapur (11768105), Samuel S. Hunter (10099278), Rene L. Suleiman (11768108), Maura C. Ruyechan (11768111), Wesley Huang (6373970), Charles G. Barbieri (11768114), Hannah W. Miller (3716773), Tammie S. Y. Tam (11768117), Matthew L. Settles (9019334), Katherine S. Ralston (11768120)

مصطلحات موضوعية: Medicine, Microbiology, Genetics, Molecular Biology, Developmental Biology, Space Science, Environmental Sciences not elsewhere classified, Biological Sciences not elsewhere classified, Physical Sciences not elsewhere classified, uncovering functional information, uncovered new details, quantify gene fragments, globally important pathogen, div >< p, defining functional domains, novel analysis pipeline, establishing forward genetics, enable forward genetics, based forward genetics, lacked annotated domains, annotated functions consistent, studies dramatically improve, identified slow growth, first rnai screen, entamoeba histolytica <, among genes targeted, sg2 mutants could, forward genetics, histolytica <, targeted genes

Relation: https://figshare.com/articles/figure/Gating_scheme_for_imaging_flow_cytometry_analysis_/17095931

الاتاحة: https://doi.org/10.1371/journal.ppat.1010088.s007

المؤلفون: Akhila Bettadapur (11768105), Samuel S. Hunter (10099278), Rene L. Suleiman (11768108), Maura C. Ruyechan (11768111), Wesley Huang (6373970), Charles G. Barbieri (11768114), Hannah W. Miller (3716773), Tammie S. Y. Tam (11768117), Matthew L. Settles (9019334), Katherine S. Ralston (11768120)

مصطلحات موضوعية: Medicine, Microbiology, Genetics, Molecular Biology, Developmental Biology, Space Science, Environmental Sciences not elsewhere classified, Biological Sciences not elsewhere classified, Physical Sciences not elsewhere classified, uncovering functional information, uncovered new details, quantify gene fragments, globally important pathogen, div >< p, defining functional domains, novel analysis pipeline, establishing forward genetics, enable forward genetics, based forward genetics, lacked annotated domains, annotated functions consistent, studies dramatically improve, identified slow growth, first rnai screen, entamoeba histolytica <, among genes targeted, sg2 mutants could, forward genetics, histolytica <, targeted genes

Relation: https://figshare.com/articles/figure/Annotated_genes_that_were_missed_or_were_least_covered_by_sequencing_reads_/17095925

الاتاحة: https://doi.org/10.1371/journal.ppat.1010088.s005

المؤلفون: Akhila Bettadapur (11768105), Samuel S. Hunter (10099278), Rene L. Suleiman (11768108), Maura C. Ruyechan (11768111), Wesley Huang (6373970), Charles G. Barbieri (11768114), Hannah W. Miller (3716773), Tammie S. Y. Tam (11768117), Matthew L. Settles (9019334), Katherine S. Ralston (11768120)

مصطلحات موضوعية: Medicine, Microbiology, Genetics, Molecular Biology, Developmental Biology, Space Science, Environmental Sciences not elsewhere classified, Biological Sciences not elsewhere classified, Physical Sciences not elsewhere classified, uncovering functional information, uncovered new details, quantify gene fragments, globally important pathogen, div >< p, defining functional domains, novel analysis pipeline, establishing forward genetics, enable forward genetics, based forward genetics, lacked annotated domains, annotated functions consistent, studies dramatically improve, identified slow growth, first rnai screen, entamoeba histolytica <, among genes targeted, sg2 mutants could, forward genetics, histolytica <, targeted genes

Relation: https://figshare.com/articles/figure/Reproducible_fragment_and_read_counts_from_biological_and_technical_replicates_/17095922

الاتاحة: https://doi.org/10.1371/journal.ppat.1010088.s004

المؤلفون: Akhila Bettadapur (11768105), Samuel S. Hunter (10099278), Rene L. Suleiman (11768108), Maura C. Ruyechan (11768111), Wesley Huang (6373970), Charles G. Barbieri (11768114), Hannah W. Miller (3716773), Tammie S. Y. Tam (11768117), Matthew L. Settles (9019334), Katherine S. Ralston (11768120)

مصطلحات موضوعية: Medicine, Microbiology, Genetics, Molecular Biology, Developmental Biology, Space Science, Environmental Sciences not elsewhere classified, Biological Sciences not elsewhere classified, Physical Sciences not elsewhere classified, uncovering functional information, uncovered new details, quantify gene fragments, globally important pathogen, div >< p, defining functional domains, novel analysis pipeline, establishing forward genetics, enable forward genetics, based forward genetics, lacked annotated domains, annotated functions consistent, studies dramatically improve, identified slow growth, first rnai screen, entamoeba histolytica <, among genes targeted, sg2 mutants could, forward genetics, histolytica <, targeted genes

Relation: https://figshare.com/articles/figure/Image_analysis_for_FLAG-SG1_transfectants_demonstrates_that_FLAG_staining_is_above_background_in_permeabilized_cells_only_/17095940

الاتاحة: https://doi.org/10.1371/journal.ppat.1010088.s010

المؤلفون: Akhila Bettadapur (11768105), Samuel S. Hunter (10099278), Rene L. Suleiman (11768108), Maura C. Ruyechan (11768111), Wesley Huang (6373970), Charles G. Barbieri (11768114), Hannah W. Miller (3716773), Tammie S. Y. Tam (11768117), Matthew L. Settles (9019334), Katherine S. Ralston (11768120)

مصطلحات موضوعية: Medicine, Microbiology, Genetics, Molecular Biology, Developmental Biology, Space Science, Environmental Sciences not elsewhere classified, Biological Sciences not elsewhere classified, Physical Sciences not elsewhere classified, uncovering functional information, uncovered new details, quantify gene fragments, globally important pathogen, div >< p, defining functional domains, novel analysis pipeline, establishing forward genetics, enable forward genetics, based forward genetics, lacked annotated domains, annotated functions consistent, studies dramatically improve, identified slow growth, first rnai screen, entamoeba histolytica <, among genes targeted, sg2 mutants could, forward genetics, histolytica <, targeted genes

Relation: https://figshare.com/articles/figure/Image_analysis_for_FLAG-SG1_transfectants_demonstrates_that_FLAG_staining_is_above_background_in_both_permeabilized_and_non-permeabilized_cells_/17095934

الاتاحة: https://doi.org/10.1371/journal.ppat.1010088.s008

المؤلفون: Akhila Bettadapur (11768105), Samuel S. Hunter (10099278), Rene L. Suleiman (11768108), Maura C. Ruyechan (11768111), Wesley Huang (6373970), Charles G. Barbieri (11768114), Hannah W. Miller (3716773), Tammie S. Y. Tam (11768117), Matthew L. Settles (9019334), Katherine S. Ralston (11768120)

مصطلحات موضوعية: Medicine, Microbiology, Genetics, Molecular Biology, Developmental Biology, Space Science, Environmental Sciences not elsewhere classified, Biological Sciences not elsewhere classified, Physical Sciences not elsewhere classified, uncovering functional information, uncovered new details, quantify gene fragments, globally important pathogen, div >< p, defining functional domains, novel analysis pipeline, establishing forward genetics, enable forward genetics, based forward genetics, lacked annotated domains, annotated functions consistent, studies dramatically improve, identified slow growth, first rnai screen, entamoeba histolytica <, among genes targeted, sg2 mutants could, forward genetics, histolytica <, targeted genes

Relation: https://figshare.com/articles/figure/Gal_GalNAc_lectin_immunofluorescence_and_image_analysis_/17095937

الاتاحة: https://doi.org/10.1371/journal.ppat.1010088.s009

المؤلفون: Akhila Bettadapur (11768105), Samuel S. Hunter (10099278), Rene L. Suleiman (11768108), Maura C. Ruyechan (11768111), Wesley Huang (6373970), Charles G. Barbieri (11768114), Hannah W. Miller (3716773), Tammie S. Y. Tam (11768117), Matthew L. Settles (9019334), Katherine S. Ralston (11768120)

مصطلحات موضوعية: Medicine, Microbiology, Genetics, Molecular Biology, Developmental Biology, Space Science, Environmental Sciences not elsewhere classified, Biological Sciences not elsewhere classified, Physical Sciences not elsewhere classified, uncovering functional information, uncovered new details, quantify gene fragments, globally important pathogen, div >< p, defining functional domains, novel analysis pipeline, establishing forward genetics, enable forward genetics, based forward genetics, lacked annotated domains, annotated functions consistent, studies dramatically improve, identified slow growth, first rnai screen, entamoeba histolytica <, among genes targeted, sg2 mutants could, forward genetics, histolytica <, targeted genes

Relation: https://figshare.com/articles/figure/Nuclear_cross-reactivity_of_the_mouse_anti-FLAG_antibody_is_only_seen_in_permeabilized_cells_/17095943

الاتاحة: https://doi.org/10.1371/journal.ppat.1010088.s011

المؤلفون: Akhila Bettadapur (11768105), Samuel S. Hunter (10099278), Rene L. Suleiman (11768108), Maura C. Ruyechan (11768111), Wesley Huang (6373970), Charles G. Barbieri (11768114), Hannah W. Miller (3716773), Tammie S. Y. Tam (11768117), Matthew L. Settles (9019334), Katherine S. Ralston (11768120)

مصطلحات موضوعية: Medicine, Microbiology, Genetics, Molecular Biology, Developmental Biology, Space Science, Environmental Sciences not elsewhere classified, Biological Sciences not elsewhere classified, Physical Sciences not elsewhere classified, uncovering functional information, uncovered new details, quantify gene fragments, globally important pathogen, div >< p, defining functional domains, novel analysis pipeline, establishing forward genetics, enable forward genetics, based forward genetics, lacked annotated domains, annotated functions consistent, studies dramatically improve, identified slow growth, first rnai screen, entamoeba histolytica <, among genes targeted, sg2 mutants could, forward genetics, histolytica <, targeted genes

Relation: https://figshare.com/articles/figure/Identification_of_slow_growing_RNAi_knockdown_mutants_/17095946

الاتاحة: https://doi.org/10.1371/journal.ppat.1010088.s012

المؤلفون: Akhila Bettadapur (11768105), Samuel S. Hunter (10099278), Rene L. Suleiman (11768108), Maura C. Ruyechan (11768111), Wesley Huang (6373970), Charles G. Barbieri (11768114), Hannah W. Miller (3716773), Tammie S. Y. Tam (11768117), Matthew L. Settles (9019334), Katherine S. Ralston (11768120)

مصطلحات موضوعية: Medicine, Microbiology, Genetics, Molecular Biology, Developmental Biology, Space Science, Environmental Sciences not elsewhere classified, Biological Sciences not elsewhere classified, Physical Sciences not elsewhere classified, uncovering functional information, uncovered new details, quantify gene fragments, globally important pathogen, div >< p, defining functional domains, novel analysis pipeline, establishing forward genetics, enable forward genetics, based forward genetics, lacked annotated domains, annotated functions consistent, studies dramatically improve, identified slow growth, first rnai screen, entamoeba histolytica <, among genes targeted, sg2 mutants could, forward genetics, histolytica <, targeted genes

Relation: https://figshare.com/articles/figure/Expression_data_for_annotated_genes_that_were_missing_in_the_final_plasmid_library_/17095952

الاتاحة: https://doi.org/10.1371/journal.ppat.1010088.s014

المؤلفون: Akhila Bettadapur (11768105), Samuel S. Hunter (10099278), Rene L. Suleiman (11768108), Maura C. Ruyechan (11768111), Wesley Huang (6373970), Charles G. Barbieri (11768114), Hannah W. Miller (3716773), Tammie S. Y. Tam (11768117), Matthew L. Settles (9019334), Katherine S. Ralston (11768120)

مصطلحات موضوعية: Medicine, Microbiology, Genetics, Molecular Biology, Developmental Biology, Space Science, Environmental Sciences not elsewhere classified, Biological Sciences not elsewhere classified, Physical Sciences not elsewhere classified, uncovering functional information, uncovered new details, quantify gene fragments, globally important pathogen, div >< p, defining functional domains, novel analysis pipeline, establishing forward genetics, enable forward genetics, based forward genetics, lacked annotated domains, annotated functions consistent, studies dramatically improve, identified slow growth, first rnai screen, entamoeba histolytica <, among genes targeted, sg2 mutants could, forward genetics, histolytica <, targeted genes

Relation: https://figshare.com/articles/figure/Sequencing_and_fragment_count_statistics_/17095949

الاتاحة: https://doi.org/10.1371/journal.ppat.1010088.s013

المؤلفون: Akhila Bettadapur (11768105), Samuel S. Hunter (10099278), Rene L. Suleiman (11768108), Maura C. Ruyechan (11768111), Wesley Huang (6373970), Charles G. Barbieri (11768114), Hannah W. Miller (3716773), Tammie S. Y. Tam (11768117), Matthew L. Settles (9019334), Katherine S. Ralston (11768120)

مصطلحات موضوعية: Medicine, Microbiology, Genetics, Molecular Biology, Developmental Biology, Space Science, Environmental Sciences not elsewhere classified, Biological Sciences not elsewhere classified, Physical Sciences not elsewhere classified, uncovering functional information, uncovered new details, quantify gene fragments, globally important pathogen, div >< p, defining functional domains, novel analysis pipeline, establishing forward genetics, enable forward genetics, based forward genetics, lacked annotated domains, annotated functions consistent, studies dramatically improve, identified slow growth, first rnai screen, entamoeba histolytica <, among genes targeted, sg2 mutants could, forward genetics, histolytica <, targeted genes

Relation: https://figshare.com/articles/figure/Primer_sequences_/17095958

الاتاحة: https://doi.org/10.1371/journal.ppat.1010088.s016

المؤلفون: Akhila Bettadapur (11768105), Samuel S. Hunter (10099278), Rene L. Suleiman (11768108), Maura C. Ruyechan (11768111), Wesley Huang (6373970), Charles G. Barbieri (11768114), Hannah W. Miller (3716773), Tammie S. Y. Tam (11768117), Matthew L. Settles (9019334), Katherine S. Ralston (11768120)

مصطلحات موضوعية: Medicine, Microbiology, Genetics, Molecular Biology, Developmental Biology, Space Science, Environmental Sciences not elsewhere classified, Biological Sciences not elsewhere classified, Physical Sciences not elsewhere classified, uncovering functional information, uncovered new details, quantify gene fragments, globally important pathogen, div >< p, defining functional domains, novel analysis pipeline, establishing forward genetics, enable forward genetics, based forward genetics, lacked annotated domains, annotated functions consistent, studies dramatically improve, identified slow growth, first rnai screen, entamoeba histolytica <, among genes targeted, sg2 mutants could, forward genetics, histolytica <, targeted genes

Relation: https://figshare.com/articles/figure/Expression_data_for_representative_genes_present_in_the_final_plasmid_library_/17095955

الاتاحة: https://doi.org/10.1371/journal.ppat.1010088.s015

المؤلفون: Akhila Bettadapur (11768105), Samuel S. Hunter (10099278), Rene L. Suleiman (11768108), Maura C. Ruyechan (11768111), Wesley Huang (6373970), Charles G. Barbieri (11768114), Hannah W. Miller (3716773), Tammie S. Y. Tam (11768117), Matthew L. Settles (9019334), Katherine S. Ralston (11768120)

مصطلحات موضوعية: Medicine, Microbiology, Genetics, Molecular Biology, Developmental Biology, Space Science, Environmental Sciences not elsewhere classified, Biological Sciences not elsewhere classified, Physical Sciences not elsewhere classified, uncovering functional information, uncovered new details, quantify gene fragments, globally important pathogen, div >< p, defining functional domains, novel analysis pipeline, establishing forward genetics, enable forward genetics, based forward genetics, lacked annotated domains, annotated functions consistent, studies dramatically improve, identified slow growth, first rnai screen, entamoeba histolytica <, among genes targeted, sg2 mutants could, forward genetics, histolytica <, targeted genes

Relation: https://figshare.com/articles/figure/Fragment_directionality_and_coverage_analysis_/17095970

الاتاحة: https://doi.org/10.1371/journal.ppat.1010088.g004

المؤلفون: Akhila Bettadapur (11768105), Samuel S. Hunter (10099278), Rene L. Suleiman (11768108), Maura C. Ruyechan (11768111), Wesley Huang (6373970), Charles G. Barbieri (11768114), Hannah W. Miller (3716773), Tammie S. Y. Tam (11768117), Matthew L. Settles (9019334), Katherine S. Ralston (11768120)

مصطلحات موضوعية: Medicine, Microbiology, Genetics, Molecular Biology, Developmental Biology, Space Science, Environmental Sciences not elsewhere classified, Biological Sciences not elsewhere classified, Physical Sciences not elsewhere classified, uncovering functional information, uncovered new details, quantify gene fragments, globally important pathogen, div >< p, defining functional domains, novel analysis pipeline, establishing forward genetics, enable forward genetics, based forward genetics, lacked annotated domains, annotated functions consistent, studies dramatically improve, identified slow growth, first rnai screen, entamoeba histolytica <, among genes targeted, sg2 mutants could, forward genetics, histolytica <, targeted genes

Relation: https://figshare.com/articles/figure/Identification_of_slow_growth_mutants_with_phenotypes_that_are_reproducible_in_independently-generated_knockdown_mutants_/17095973

الاتاحة: https://doi.org/10.1371/journal.ppat.1010088.g005

المؤلفون: Akhila Bettadapur (11768105), Samuel S. Hunter (10099278), Rene L. Suleiman (11768108), Maura C. Ruyechan (11768111), Wesley Huang (6373970), Charles G. Barbieri (11768114), Hannah W. Miller (3716773), Tammie S. Y. Tam (11768117), Matthew L. Settles (9019334), Katherine S. Ralston (11768120)

مصطلحات موضوعية: Medicine, Microbiology, Genetics, Molecular Biology, Developmental Biology, Space Science, Environmental Sciences not elsewhere classified, Biological Sciences not elsewhere classified, Physical Sciences not elsewhere classified, uncovering functional information, uncovered new details, quantify gene fragments, globally important pathogen, div >< p, defining functional domains, novel analysis pipeline, establishing forward genetics, enable forward genetics, based forward genetics, lacked annotated domains, annotated functions consistent, studies dramatically improve, identified slow growth, first rnai screen, entamoeba histolytica <, among genes targeted, sg2 mutants could, forward genetics, histolytica <, targeted genes

Relation: https://figshare.com/articles/figure/RNAi_library_construction_/17095964

الاتاحة: https://doi.org/10.1371/journal.ppat.1010088.g002

المؤلفون: Akhila Bettadapur (11768105), Samuel S. Hunter (10099278), Rene L. Suleiman (11768108), Maura C. Ruyechan (11768111), Wesley Huang (6373970), Charles G. Barbieri (11768114), Hannah W. Miller (3716773), Tammie S. Y. Tam (11768117), Matthew L. Settles (9019334), Katherine S. Ralston (11768120)

مصطلحات موضوعية: Medicine, Microbiology, Genetics, Molecular Biology, Developmental Biology, Space Science, Environmental Sciences not elsewhere classified, Biological Sciences not elsewhere classified, Physical Sciences not elsewhere classified, uncovering functional information, uncovered new details, quantify gene fragments, globally important pathogen, div >< p, defining functional domains, novel analysis pipeline, establishing forward genetics, enable forward genetics, based forward genetics, lacked annotated domains, annotated functions consistent, studies dramatically improve, identified slow growth, first rnai screen, entamoeba histolytica <, among genes targeted, sg2 mutants could, forward genetics, histolytica <, targeted genes

Relation: https://figshare.com/articles/figure/The_final_RNAi_library_has_genome-wide_coverage_/17095967

الاتاحة: https://doi.org/10.1371/journal.ppat.1010088.g003