يعرض 1 - 20 نتائج من 115 نتيجة بحث عن '"GC–MS: Gas chromatography-mass spectrometry"', وقت الاستعلام: 0.65s تنقيح النتائج
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    المؤلفون: Matthew James Carlo, Amanda Patrick

    المصدر: Journal of Mass Spectrometry and Advances in the Clinical Lab, Vol 23, Iss, Pp 14-25 (2022)
    Journal of Mass Spectrometry and Advances in the Clinical Lab

    مصطلحات موضوعية: GCMS, gas chromatography-mass spectrometry, Vibrational spectroscopy, LC-MS, liquid chromatography-mass spectrometry, MS/MS, tandem mass spectrometry, GSNO, S- nitro glutathione, PTM, post-translational modification, FA, fluoroamphetamine, UV-IR, ultraviolet-infrared, FMA, fluoromethamphetamine, Clinical Biochemistry, DFT, density functional theory, FTICR, Fourier transform ion cyclotron resonance, CLIO, Centre Laser Infrarouge d’Orsay, Microbiology, Infrared multiple photon dissociation spectroscopy, NANT, N-acetyl-N-nitrosotryptophan, IR, infrared, SNOCys, S-nitrosocysteine, 2P1EA, 2-phenyl-1-ethanolamine, IRPD, infrared predissociation spectroscopy, LC-MS/MS, liquid chromatography-tandem mass spectrometry, Metabolites, Medical technology, IRMPD, infrared multiple photon dissociation (IRMPD), IVR, intramolecular vibrational redistribution, Advances in MS Instrumentation: The Present and Future of the Clinical Lab, R855-855.5, Spectroscopy, cw, continuous wave, MDA, methylenedioxyamphetamine, ComputingMethodologies_COMPUTERGRAPHICS, OPO/A, optical parametric oscillator/amplifier, Mass spectrometry, LC, liquid chromatography, CIVP, cryogenic ion vibrational predissociation spectroscopy, IR2MS3, infrared-infrared double-resonance multi-stage mass spectrometry, 2-AEP, 2-aminoethylphosphonic acid, MSn, multi-stage mass spectrometry, UV, ultraviolet, FELIX, Free Electron Laser for Infrared eXperiments, Medical Laboratory Technology, α-PVP, alpha-pyrrolidinovalerophenone, FEL, free electron laser, IRMPD-MS, infrared multiple photon dissociation spectroscopy mass spectrometry, Pharmaceuticals, GlcNAc, n-Acetylglucosamine, MMC, methylmethcathinone, MDMA, methylenedioxymethamphetamine, Post-translational modifications

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    المصدر: Saudi Journal of Biological Sciences
    Saudi Journal of Biological Sciences, Vol 28, Iss 12, Pp 7082-7089 (2021)

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    المصدر: Saudi Journal of Biological Sciences
    Saudi Journal of Biological Sciences, Vol 28, Iss 5, Pp 2877-2885 (2021)

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    المصدر: Computational and Structural Biotechnology Journal
    Computational and Structural Biotechnology Journal, Vol 19, Iss, Pp 1956-1965 (2021)

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    المصدر: Journal of Mass Spectrometry and Advances in the Clinical Lab
    Journal of Mass Spectrometry and Advances in the Clinical Lab, Vol 19, Iss, Pp 34-45 (2021)

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    Academic Journal

    المساهمون: C. Fattuoni, C. Mandò, F. Palma, G.M. Anelli, C. Novielli, E. Parejo Laudicina, V.M. Savasi, L. Barberini, A. Dessì, R. Pintu, V. Fano, A. Noto, I. Cetin

    مصطلحات موضوعية: INTRODUCTION: The placenta plays a pivotal role as it represents the connection between mother and foetus. It acts as a carrier (nutrients and oxygen, waste products and carbonic anhydride) as well as a barrier against infection. Pregnancy related metabolomics has examined different biofluids in order to reach deeper insights into pregnancy pathologies [1]. Amniotic fluid, urine and plasma were the most studied, while placenta tissue has been the subject of few studies [2]. In this report, a method to analyse the placental metabolome through the GC-MS (Gas Chromatography-Mass Spectrometry) technique is described. MATERIALS AND METHODS: Placenta tissues were collected after elective caesarean section from obese and normal weight mothers, snap frozen and stored at -80°C until analysis. Each sample was homogenized with a mixture of solvents (chloroform, methanol, water), centrifuged, and the obtained phases were separated and processed following two different protocols. The hydrophilic phase was vacuum dried and treated with two derivatizing reagents, methoxyamine hydrochloride and MSTFA (N-Methyl-N-[trimethylsilyl] trifluoroacetamide), to give a mixture of trimethylsilylated metabolites. The lipophilic phase was dried and treated with boron trifluoride in methanol to obtain a mixture of FAME (Fatty Acid Methyl Esters). The two phases were separately analysed on a GC-MS platform, applying different instrumental conditions. The chromatograms obtained were analysed with the free software AMDIS (Automated Mass Spectral Deconvolution and Identification System) using a lab-made library comprising 200 metabolites. RESULTS: The analysis of the placenta tissue allowed to identify essential metabolites. In the hydrophilic fraction, 78 metabolites were detected: they mainly belong to the classes of amino acids (18), carboxylic acids (13), sugars and sugar-related compounds (11), phosphorylated derivatives (9). The lipophilic fraction analysis allowed to define the fatty acid profile of the placenta extract: the saturated fatty acids palmitic and stearic, the monounsaturated oleic, and the polyunsaturated (PUFA) linoleic, arachidonic, 8,11,14-eicosatrienoic and 4,7,10,13,16,19-docosahexaenoic acid (DHA) were the most representative. Statistical analysis of the data matrices derived from the hydrophilic and lipophilic fraction analysis were performed in MetaboAnalyst 3.0 [3], to investigate the possibility of identifying characteristic metabolic differences between the different phenotypes. Placentae from obese mothers were compared with those from normal weight mothers to get new insights into the correlation between the metabolite content and metabolic status. CONCLUSIONS: The reported analysis method allowed to examine different metabolites in placenta samples, divided into two phases, hydrophilic and lipophilic. All metabolites were analysed through the same GC-MS platform to provide interesting informations about the placenta tissue composition. REFERENCES [1] Fanos V, Atzori L, Makarenko K, Melis GB, Ferrazzi E. Metabolomics application in maternal-fetal medicine. Biomed Res Int. 2013, 2013: 720514. [2] Dunn WB, Brown M

    Relation: International Workshop on Neonatology; volume:6; issue:2; firstpage:81; lastpage:82; numberofpages:2; journal:JOURNAL OF PEDIATRIC AND NEONATAL INDIVIDUALIZED MEDICINE; http://hdl.handle.net/2434/545220

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    المصدر: Current Research in Toxicology, Vol 1, Iss, Pp 133-140 (2020)
    Current Research in Toxicology

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    Academic Journal

    المؤلفون: Özbay, Sena, Şireli, Ufuk Tansel

    المساهمون: Rektörlük, orcid:0000-0001-6024-0805

    وصف الملف: application/pdf

    Relation: Journal of Food Science and Technology; Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı; 0022-1155 / 0975-8402; https:/dx.doi.org/10.1007/s13197-021-05194-2; https://hdl.handle.net/20.500.12451/8565

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