المؤلفون: Laura Leoni (11495393), Francesca Tonelli (11495396), Roberta Besio (389022), Roberta Gioia (389023), Francesco Moccia (133351), Antonio Rossi (184985), Antonella Forlino (389028)

مصطلحات موضوعية: Microbiology, Genetics, Molecular Biology, Biological Sciences not elsewhere classified, technique widely applicable, patients ’ osteoblasts, patients ’ cells, fully take advantage, div >< p, ko clones unveiled, vivo <, vitro <, variable degree, use make, ubiquitously present, type xiv, tmem38b <, three grnas, severity associated, powerful technology, osteogenesis imperfecta, limited cost, largely unknown, impaired secretion, impaired proliferation, heritable disorders, endoplasmic reticulum

Relation: https://figshare.com/articles/journal_contribution/S1_Materials_and_methods_-/16693745

الاتاحة: https://doi.org/10.1371/journal.pone.0257254.s001

المؤلفون: Laura Leoni (11495393), Francesca Tonelli (11495396), Roberta Besio (389022), Roberta Gioia (389023), Francesco Moccia (133351), Antonio Rossi (184985), Antonella Forlino (389028)

مصطلحات موضوعية: Microbiology, Genetics, Molecular Biology, Biological Sciences not elsewhere classified, technique widely applicable, patients ’ osteoblasts, patients ’ cells, fully take advantage, div >< p, ko clones unveiled, vivo <, vitro <, variable degree, use make, ubiquitously present, type xiv, tmem38b <, three grnas, severity associated, powerful technology, osteogenesis imperfecta, limited cost, largely unknown, impaired secretion, impaired proliferation, heritable disorders, endoplasmic reticulum

Relation: https://figshare.com/articles/journal_contribution/S1_Results_-/16693748

الاتاحة: https://doi.org/10.1371/journal.pone.0257254.s002

المؤلفون: Laura Leoni (11495393), Francesca Tonelli (11495396), Roberta Besio (389022), Roberta Gioia (389023), Francesco Moccia (133351), Antonio Rossi (184985), Antonella Forlino (389028)

مصطلحات موضوعية: Microbiology, Genetics, Molecular Biology, Biological Sciences not elsewhere classified, technique widely applicable, patients ’ osteoblasts, patients ’ cells, fully take advantage, div >< p, ko clones unveiled, vivo <, vitro <, variable degree, use make, ubiquitously present, type xiv, tmem38b <, three grnas, severity associated, powerful technology, osteogenesis imperfecta, limited cost, largely unknown, impaired secretion, impaired proliferation, heritable disorders, endoplasmic reticulum

Relation: https://figshare.com/articles/journal_contribution/Mutant_clonal_lines_screening_by_T7_endonuclease_I_assay_and_restriction_enzyme_digestions_/16693754

الاتاحة: https://doi.org/10.1371/journal.pone.0257254.s004

المؤلفون: Laura Leoni (11495393), Francesca Tonelli (11495396), Roberta Besio (389022), Roberta Gioia (389023), Francesco Moccia (133351), Antonio Rossi (184985), Antonella Forlino (389028)

مصطلحات موضوعية: Microbiology, Genetics, Molecular Biology, Biological Sciences not elsewhere classified, technique widely applicable, patients ’ osteoblasts, patients ’ cells, fully take advantage, div >< p, ko clones unveiled, vivo <, vitro <, variable degree, use make, ubiquitously present, type xiv, tmem38b <, three grnas, severity associated, powerful technology, osteogenesis imperfecta, limited cost, largely unknown, impaired secretion, impaired proliferation, heritable disorders, endoplasmic reticulum

Relation: https://figshare.com/articles/journal_contribution/Screening_of_hFOB_transfected_with_the_pSpCas9_BB_-2A-GFP_and_pSpCas9_BB_-2A-GFP-gRNA-2_vectors_/16693751

الاتاحة: https://doi.org/10.1371/journal.pone.0257254.s003

المؤلفون: Laura Leoni (11495393), Francesca Tonelli (11495396), Roberta Besio (389022), Roberta Gioia (389023), Francesco Moccia (133351), Antonio Rossi (184985), Antonella Forlino (389028)

مصطلحات موضوعية: Microbiology, Genetics, Molecular Biology, Biological Sciences not elsewhere classified, technique widely applicable, patients ’ osteoblasts, patients ’ cells, fully take advantage, div >< p, ko clones unveiled, vivo <, vitro <, variable degree, use make, ubiquitously present, type xiv, tmem38b <, three grnas, severity associated, powerful technology, osteogenesis imperfecta, limited cost, largely unknown, impaired secretion, impaired proliferation, heritable disorders, endoplasmic reticulum

Relation: https://figshare.com/articles/journal_contribution/Original_uncropped_scans_of_the_western_blots_shown_in_Fig_i_1B_i_and_Fig_i_3_i_C_/16693757

الاتاحة: https://doi.org/10.1371/journal.pone.0257254.s005

المؤلفون: Laura Leoni (11495393), Francesca Tonelli (11495396), Roberta Besio (389022), Roberta Gioia (389023), Francesco Moccia (133351), Antonio Rossi (184985), Antonella Forlino (389028)

مصطلحات موضوعية: Microbiology, Genetics, Molecular Biology, Biological Sciences not elsewhere classified, technique widely applicable, patients ’ osteoblasts, patients ’ cells, fully take advantage, div >< p, ko clones unveiled, vivo <, vitro <, variable degree, use make, ubiquitously present, type xiv, tmem38b <, three grnas, severity associated, powerful technology, osteogenesis imperfecta, limited cost, largely unknown, impaired secretion, impaired proliferation, heritable disorders, endoplasmic reticulum

Relation: https://figshare.com/articles/journal_contribution/Ca_sup_2_sup_mobilization_from_ER_/16693760

الاتاحة: https://doi.org/10.1371/journal.pone.0257254.s006

المؤلفون: Laura Leoni (11495393), Francesca Tonelli (11495396), Roberta Besio (389022), Roberta Gioia (389023), Francesco Moccia (133351), Antonio Rossi (184985), Antonella Forlino (389028)

مصطلحات موضوعية: Microbiology, Genetics, Molecular Biology, Biological Sciences not elsewhere classified, technique widely applicable, patients ’ osteoblasts, patients ’ cells, fully take advantage, div >< p, ko clones unveiled, vivo <, vitro <, variable degree, use make, ubiquitously present, type xiv, tmem38b <, three grnas, severity associated, powerful technology, osteogenesis imperfecta, limited cost, largely unknown, impaired secretion, impaired proliferation, heritable disorders, endoplasmic reticulum

Relation: https://figshare.com/articles/journal_contribution/Validation_of_further_i_TMEM38B_i_KO_hFOBs_/16693763

الاتاحة: https://doi.org/10.1371/journal.pone.0257254.s007

المؤلفون: Laura Leoni (11495393), Francesca Tonelli (11495396), Roberta Besio (389022), Roberta Gioia (389023), Francesco Moccia (133351), Antonio Rossi (184985), Antonella Forlino (389028)

مصطلحات موضوعية: Microbiology, Genetics, Molecular Biology, Biological Sciences not elsewhere classified, technique widely applicable, patients ’ osteoblasts, patients ’ cells, fully take advantage, div >< p, ko clones unveiled, vivo <, vitro <, variable degree, use make, ubiquitously present, type xiv, tmem38b <, three grnas, severity associated, powerful technology, osteogenesis imperfecta, limited cost, largely unknown, impaired secretion, impaired proliferation, heritable disorders, endoplasmic reticulum

Relation: https://figshare.com/articles/journal_contribution/Expression_of_osteoblast_markers_and_osteoblast_proliferation_upon_inhibition_of_Ca_sup_2_sup_flux_by_2-APB_/16693766

الاتاحة: https://doi.org/10.1371/journal.pone.0257254.s008

المؤلفون: Laura Leoni (11495393), Francesca Tonelli (11495396), Roberta Besio (389022), Roberta Gioia (389023), Francesco Moccia (133351), Antonio Rossi (184985), Antonella Forlino (389028)

مصطلحات موضوعية: Microbiology, Genetics, Molecular Biology, Biological Sciences not elsewhere classified, technique widely applicable, patients ’ osteoblasts, patients ’ cells, fully take advantage, div >< p, ko clones unveiled, vivo <, vitro <, variable degree, use make, ubiquitously present, type xiv, tmem38b <, three grnas, severity associated, powerful technology, osteogenesis imperfecta, limited cost, largely unknown, impaired secretion, impaired proliferation, heritable disorders, endoplasmic reticulum

Relation: https://figshare.com/articles/journal_contribution/Enzyme_digestion_for_clones_transfected_with_gRNA2_/16693769

الاتاحة: https://doi.org/10.1371/journal.pone.0257254.s009

المؤلفون: Laura Leoni (11495393), Francesca Tonelli (11495396), Roberta Besio (389022), Roberta Gioia (389023), Francesco Moccia (133351), Antonio Rossi (184985), Antonella Forlino (389028)

مصطلحات موضوعية: Microbiology, Genetics, Molecular Biology, Biological Sciences not elsewhere classified, technique widely applicable, patients ’ osteoblasts, patients ’ cells, fully take advantage, div >< p, ko clones unveiled, vivo <, vitro <, variable degree, use make, ubiquitously present, type xiv, tmem38b <, three grnas, severity associated, powerful technology, osteogenesis imperfecta, limited cost, largely unknown, impaired secretion, impaired proliferation, heritable disorders, endoplasmic reticulum

Relation: https://figshare.com/articles/journal_contribution/Enzyme_digestion_for_clones_transfected_with_gRNA3_2_/16693772

الاتاحة: https://doi.org/10.1371/journal.pone.0257254.s010

المؤلفون: Laura Leoni (11495393), Francesca Tonelli (11495396), Roberta Besio (389022), Roberta Gioia (389023), Francesco Moccia (133351), Antonio Rossi (184985), Antonella Forlino (389028)

مصطلحات موضوعية: Microbiology, Genetics, Molecular Biology, Biological Sciences not elsewhere classified, technique widely applicable, patients ’ osteoblasts, patients ’ cells, fully take advantage, div >< p, ko clones unveiled, vivo <, vitro <, variable degree, use make, ubiquitously present, type xiv, tmem38b <, three grnas, severity associated, powerful technology, osteogenesis imperfecta, limited cost, largely unknown, impaired secretion, impaired proliferation, heritable disorders, endoplasmic reticulum

Relation: https://figshare.com/articles/journal_contribution/Enzyme_digestion_for_clones_transfected_with_both_gRNA2_and_gRNA3_2_/16693775

الاتاحة: https://doi.org/10.1371/journal.pone.0257254.s011

المؤلفون: Laura Leoni (11495393), Francesca Tonelli (11495396), Roberta Besio (389022), Roberta Gioia (389023), Francesco Moccia (133351), Antonio Rossi (184985), Antonella Forlino (389028)

مصطلحات موضوعية: Microbiology, Genetics, Molecular Biology, Biological Sciences not elsewhere classified, technique widely applicable, patients ’ osteoblasts, patients ’ cells, fully take advantage, div >< p, ko clones unveiled, vivo <, vitro <, variable degree, use make, ubiquitously present, type xiv, tmem38b <, three grnas, severity associated, powerful technology, osteogenesis imperfecta, limited cost, largely unknown, impaired secretion, impaired proliferation, heritable disorders, endoplasmic reticulum

Relation: https://figshare.com/articles/journal_contribution/S1_Raw_images_-/16693778

الاتاحة: https://doi.org/10.1371/journal.pone.0257254.s012

المؤلفون: Laura Leoni (11495393), Francesca Tonelli (11495396), Roberta Besio (389022), Roberta Gioia (389023), Francesco Moccia (133351), Antonio Rossi (184985), Antonella Forlino (389028)

مصطلحات موضوعية: Microbiology, Genetics, Molecular Biology, Biological Sciences not elsewhere classified, technique widely applicable, patients ’ osteoblasts, patients ’ cells, fully take advantage, div >< p, ko clones unveiled, vivo <, vitro <, variable degree, use make, ubiquitously present, type xiv, tmem38b <, three grnas, severity associated, powerful technology, osteogenesis imperfecta, limited cost, largely unknown, impaired secretion, impaired proliferation, heritable disorders, endoplasmic reticulum

Relation: https://figshare.com/articles/figure/OI_type_XIV_i_in_vitro_i_model_generation_and_western_blot_validation_/16693781

الاتاحة: https://doi.org/10.1371/journal.pone.0257254.g001

المؤلفون: Laura Leoni (11495393), Francesca Tonelli (11495396), Roberta Besio (389022), Roberta Gioia (389023), Francesco Moccia (133351), Antonio Rossi (184985), Antonella Forlino (389028)

مصطلحات موضوعية: Microbiology, Genetics, Molecular Biology, Biological Sciences not elsewhere classified, technique widely applicable, patients ’ osteoblasts, patients ’ cells, fully take advantage, div >< p, ko clones unveiled, vivo <, vitro <, variable degree, use make, ubiquitously present, type xiv, tmem38b <, three grnas, severity associated, powerful technology, osteogenesis imperfecta, limited cost, largely unknown, impaired secretion, impaired proliferation, heritable disorders, endoplasmic reticulum

Relation: https://figshare.com/articles/figure/Expression_analysis_of_osteoblastogenic_markers_and_of_the_RANKL_OPG_ratio_/16693787

الاتاحة: https://doi.org/10.1371/journal.pone.0257254.g003

المؤلفون: Laura Leoni (11495393), Francesca Tonelli (11495396), Roberta Besio (389022), Roberta Gioia (389023), Francesco Moccia (133351), Antonio Rossi (184985), Antonella Forlino (389028)

مصطلحات موضوعية: Microbiology, Genetics, Molecular Biology, Biological Sciences not elsewhere classified, technique widely applicable, patients ’ osteoblasts, patients ’ cells, fully take advantage, div >< p, ko clones unveiled, vivo <, vitro <, variable degree, use make, ubiquitously present, type xiv, tmem38b <, three grnas, severity associated, powerful technology, osteogenesis imperfecta, limited cost, largely unknown, impaired secretion, impaired proliferation, heritable disorders, endoplasmic reticulum

Relation: https://figshare.com/articles/figure/Intracellular_Ca_sup_2_sup_concentration_ER_calcium_flux_and_collagen_analysis_/16693784

الاتاحة: https://doi.org/10.1371/journal.pone.0257254.g002

المؤلفون: Laura Leoni (11495393), Francesca Tonelli (11495396), Roberta Besio (389022), Roberta Gioia (389023), Francesco Moccia (133351), Antonio Rossi (184985), Antonella Forlino (389028)

مصطلحات موضوعية: Microbiology, Genetics, Molecular Biology, Biological Sciences not elsewhere classified, technique widely applicable, patients ’ osteoblasts, patients ’ cells, fully take advantage, div >< p, ko clones unveiled, vivo <, vitro <, variable degree, use make, ubiquitously present, type xiv, tmem38b <, three grnas, severity associated, powerful technology, osteogenesis imperfecta, limited cost, largely unknown, impaired secretion, impaired proliferation, heritable disorders, endoplasmic reticulum

Relation: https://figshare.com/articles/figure/Cell_proliferation_collagen_secretion_and_matrix_mineralization_analysis_/16693790

الاتاحة: https://doi.org/10.1371/journal.pone.0257254.g004

المؤلفون: Laura Leoni (11495393), Francesca Tonelli (11495396), Roberta Besio (389022), Roberta Gioia (389023), Francesco Moccia (133351), Antonio Rossi (184985), Antonella Forlino (389028)

مصطلحات موضوعية: Microbiology, Genetics, Molecular Biology, Biological Sciences not elsewhere classified, technique widely applicable, patients ’ osteoblasts, patients ’ cells, fully take advantage, div >< p, ko clones unveiled, vivo <, vitro <, variable degree, use make, ubiquitously present, type xiv, tmem38b <, three grnas, severity associated, powerful technology, osteogenesis imperfecta, limited cost, largely unknown, impaired secretion, impaired proliferation, heritable disorders, endoplasmic reticulum

Relation: https://figshare.com/articles/dataset/Mutations_identified_in_gRNA-2_and_gRNA-3_2_transfected_clones_/16693793

الاتاحة: https://doi.org/10.1371/journal.pone.0257254.t001