يعرض 1 - 20 نتائج من 38 نتيجة بحث عن '"Fraction Analysis"', وقت الاستعلام: 0.54s تنقيح النتائج
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    Academic Journal
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    Academic Journal

    المصدر: Sulaeva , I , Budischowsky , D , Rahikainen , J , Marjamaa , K , Støpamo , F G , Khaliliyan , H , Melikhov , I , Rosenau , T , Kruus , K , Várnai , A , Eijsink , V G H & Potthast , A 2024 , ' A novel approach to analyze the impact of lytic polysaccharide monooxygenases (LPMOs) on cellulosic fibres ' , Carbohydrate Polymers , vol. 328 , 121696 . https://doi.org/10.1016/j.carbpol.2023.121696

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    Academic Journal
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    Academic Journal
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    Academic Journal
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    Academic Journal

    المساهمون: C. Fattuoni, C. Mandò, F. Palma, G.M. Anelli, C. Novielli, E. Parejo Laudicina, V.M. Savasi, L. Barberini, A. Dessì, R. Pintu, V. Fano, A. Noto, I. Cetin

    مصطلحات موضوعية: INTRODUCTION: The placenta plays a pivotal role as it represents the connection between mother and foetus. It acts as a carrier (nutrients and oxygen, waste products and carbonic anhydride) as well as a barrier against infection. Pregnancy related metabolomics has examined different biofluids in order to reach deeper insights into pregnancy pathologies [1]. Amniotic fluid, urine and plasma were the most studied, while placenta tissue has been the subject of few studies [2]. In this report, a method to analyse the placental metabolome through the GC-MS (Gas Chromatography-Mass Spectrometry) technique is described. MATERIALS AND METHODS: Placenta tissues were collected after elective caesarean section from obese and normal weight mothers, snap frozen and stored at -80°C until analysis. Each sample was homogenized with a mixture of solvents (chloroform, methanol, water), centrifuged, and the obtained phases were separated and processed following two different protocols. The hydrophilic phase was vacuum dried and treated with two derivatizing reagents, methoxyamine hydrochloride and MSTFA (N-Methyl-N-[trimethylsilyl] trifluoroacetamide), to give a mixture of trimethylsilylated metabolites. The lipophilic phase was dried and treated with boron trifluoride in methanol to obtain a mixture of FAME (Fatty Acid Methyl Esters). The two phases were separately analysed on a GC-MS platform, applying different instrumental conditions. The chromatograms obtained were analysed with the free software AMDIS (Automated Mass Spectral Deconvolution and Identification System) using a lab-made library comprising 200 metabolites. RESULTS: The analysis of the placenta tissue allowed to identify essential metabolites. In the hydrophilic fraction, 78 metabolites were detected: they mainly belong to the classes of amino acids (18), carboxylic acids (13), sugars and sugar-related compounds (11), phosphorylated derivatives (9). The lipophilic fraction analysis allowed to define the fatty acid profile of the placenta extract: the saturated fatty acids palmitic and stearic, the monounsaturated oleic, and the polyunsaturated (PUFA) linoleic, arachidonic, 8,11,14-eicosatrienoic and 4,7,10,13,16,19-docosahexaenoic acid (DHA) were the most representative. Statistical analysis of the data matrices derived from the hydrophilic and lipophilic fraction analysis were performed in MetaboAnalyst 3.0 [3], to investigate the possibility of identifying characteristic metabolic differences between the different phenotypes. Placentae from obese mothers were compared with those from normal weight mothers to get new insights into the correlation between the metabolite content and metabolic status. CONCLUSIONS: The reported analysis method allowed to examine different metabolites in placenta samples, divided into two phases, hydrophilic and lipophilic. All metabolites were analysed through the same GC-MS platform to provide interesting informations about the placenta tissue composition. REFERENCES [1] Fanos V, Atzori L, Makarenko K, Melis GB, Ferrazzi E. Metabolomics application in maternal-fetal medicine. Biomed Res Int. 2013, 2013: 720514. [2] Dunn WB, Brown M

    Relation: International Workshop on Neonatology; volume:6; issue:2; firstpage:81; lastpage:82; numberofpages:2; journal:JOURNAL OF PEDIATRIC AND NEONATAL INDIVIDUALIZED MEDICINE; http://hdl.handle.net/2434/545220

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    Academic Journal

    وصف الملف: application/pdf

    Relation: Вестник ЮУрГУ. Серия Химия; Vestnik Ûžno-Ural’skogo gosudarstvennogo universiteta. Seriâ Himiâ; Bulletin of SUSU. Ser. Chemistry; Вестник Южно-Уральского государственного университета. Серия Химия; Bulletin of the South Ural State University. Ser. Chemistry; Химия;Том 14; Калимуллина Л.В. Деарилирование три(орто-толил)висмута мезитиленсульфоновой кислотой / Л.В. Калимуллина, В.В. Шарутин // Вестник ЮУрГУ. Серия «Химия». 2022. Т. 14, № 4. С. 55–63. DOI:10.14529/chem220405. Kalimullina L.V., Sharutin V.V. Dearylation of tri(ortho-tolyl)bismuth with mesitylenesulfonic acid. Bulletin of the South Ural State University. Ser. Chemistry. 2022;14(4):55–63. (In Russ.). DOI:10.14529/chem220405; http://dspace.susu.ru/xmlui/handle/00001.74/51162

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    Academic Journal

    المصدر: Marine Pollution Bulletin

    وصف الملف: application/pdf

    Relation: https://eprints.qut.edu.au/81980/1/Weak%20acid%20extractable%20metals%20in%20Bramble%20Bay,%20Queensland,%20Australia%20Temporal%20behaviour,%20enrichment%20and%20source%20apportionment.pdf; Brady, James, Ayoko, Godwin, Martens, Wayde, & Goonetilleke, Ashantha (2015) Weak acid extractable metals in Bramble Bay, Queensland, Australia: Temporal behaviour, enrichment and source apportionment. Marine Pollution Bulletin, 91(1), pp. 380-388.; https://eprints.qut.edu.au/81980/; Institute for Future Environments; Science & Engineering Faculty

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    Academic Journal

    المصدر: Marine Pollution Bulletin

    وصف الملف: application/pdf

    Relation: https://eprints.qut.edu.au/79646/1/Temporal%20trends%20and%20bioavailability%20assessment%20of%20heavy%20metals%20in%20the%20sediments%20of%20Deception%20Bay_Queensland,%20Australia.pdf; Brady, James, Ayoko, Godwin, Martens, Wayde, & Goonetilleke, Ashantha (2014) Temporal trends and bioavailability assessment of heavy metals in the sediments of Deception Bay, Queensland, Australia. Marine Pollution Bulletin, 89(1 - 2), pp. 464-472.; https://eprints.qut.edu.au/79646/; Institute for Future Environments; Science & Engineering Faculty

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    Dissertation/ Thesis

    المساهمون: Μαρινακης Δημητριος, Marinakis Dimitrios, Πασαδακης Νικος, Pasadakis Nikos, Γιωτης Ανδρεας, Giotis Andreas, Επιβλέπων: Γιωτης Ανδρεας, Advisor: Giotis Andreas, Μέλος επιτροπής: Μαρινακης Δημητριος, Committee member: Marinakis Dimitrios, Μέλος επιτροπής: Πασαδακης Νικος, Committee member: Pasadakis Nikos

    وصف الملف: application/pdf

    Relation: info:eu-repo/grantAgreement/EC/FP7/246686; http://purl.tuc.gr/dl/dias/06557798-745E-446C-A6DB-29667A6A9038

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    Academic Journal
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    Dissertation/ Thesis

    المؤلفون: Brady, James P.

    وصف الملف: application/pdf

    Relation: https://eprints.qut.edu.au/89686/6/89686%28thesis%29.pdf; Brady, James P. (2015) Heavy metals in the sediments of Northern Moreton Bay, Queensland, Australia. PhD by Publication, Queensland University of Technology.; https://eprints.qut.edu.au/89686/; Science & Engineering Faculty; School of Chemistry, Physics & Mechanical Engineering; School of Earth, Environmental & Biological Sciences

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    Academic Journal
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    Report

    المساهمون: Robinson, J

    المصدر: Other Information: UNCL. Orig. Receipt Date: 31-DEC-69

    وصف الملف: Medium: X; Size: Pages: 32