المؤلفون: Báez, Indira Rojo, Castro, Daniel Omar Rivera, Gutiérrez, Sarahí Salas, López, Edgar Dehesa, Lemarroy, Adriana Aguilar, Suarez, Luis Felipe Jave, Ureta, Hipólito Castillo, Montoya, Edith Hilario Torres, Andalón, Vicente Olimón

المصدر: Sao Paulo Medical Journal. March 2020 138(2)

مصطلحات موضوعية: Sfrp5 protein, human [supplementary concept], Wnt5a protein, human [supplementary concept], Diabetes mellitus, type 2, Renal insufficiency, chronic, Diabetes mellitus, Anti-inflammatory cytokine, Diabetic nephropathy, ELISA quantification, Sfrp5, Wnt5a

وصف الملف: text/html

URL الوصول: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-31802020000200133

المؤلفون: O Evrova, J Buschmann

المصدر: European Cells & Materials, Vol 34, Pp 15-39 (2017)

مصطلحات موضوعية: the range of bioactive components in dentine and specific biological effects on bone-derived mesenchymal stem cells (MSCs) in humans are less well understood. The aims of this study were to further elucidate the biological response of MSCs to demineralised dentine matrix (DDM) in enhancing wound repair responses and ascertain key contributing components. Dentine was obtained from human teeth and DDM proteins solubilised with ethylenediaminetetraacetic acid (EDTA). Bone marrow derived MSCs were commercially obtained. Cells with a more immature phenotype were then selected by preferential fibronectin adhesion (FN-BMMSCs) for use in subsequent in vitro assays. DDM at 10 µg/mL reduced cell expansion, attenuated apoptosis and was the minimal concentration capable of inducing osteoblastic differentiation. Enzyme-linked immunosorbent assay (ELISA) quantification of growth factors indicated physiological levels produced the above responses, transforming growth factor β (TGF-β1) was predominant (15.6 ng/mg DDM), with relatively lower concentrations of BMP-2, FGF, VEGF and PDGF (6.2-4.7 ng/mg DDM). Fractionation of growth factors from other DDM components by heparin affinity chromatography diminished osteogenic responses. Depletion of biglycan from DDM also attenuated osteogenic potency, which was partially rescued by the isolated biglycan. Decorin depletion from DDM had no influence on osteogenic potency. Collectively, these results demonstrate the potential of DDM for the delivery of physiological levels of growth factors for bone repair processes, Mesenchymal stem cells, dentine, growth factors, biglycan, decorin, cell expansion, osteoblast differentiation, migration, apoptosis, Diseases of the musculoskeletal system, RC925-935, Orthopedic surgery, RD701-811

وصف الملف: electronic resource

Relation: http://www.ecmjournal.org/papers/vol034/pdf/v034a02.pdf; https://doaj.org/toc/1473-2262

URL الوصول: https://doaj.org/article/5470f7650d56439dad0fee05808bc9d0

المؤلفون: Sarahí Salas Gutiérrez, Edgar Dehesa López, Indira Rojo Báez, Hipólito Castillo Ureta, Luis Felipe Jave Suarez, Adriana Aguilar Lemarroy, Vicente Olimón Andalón, Daniel Omar Rivera Castro, Edith Hilario Torres Montoya

المصدر: Sao Paulo Medical Journal, Volume: 138, Issue: 2, Pages: 133-139, Published: 01 JUN 2020
São Paulo Medical Journal, Vol 138, Iss 2, Pp 133-139
Sao Paulo Medical Journal v.138 n.2 2020
São Paulo medical journal
Associação Paulista de Medicina
instacron:APM

مصطلحات موضوعية: anti-inflammatory cytokine, sfrp5 protein, human [supplementary concept], lcsh:Medicine, Diabetic nephropathy, Gastroenterology, 0302 clinical medicine, Diabetes mellitus, Sfrp5, 030212 general & internal medicine, ELISA quantification, Intracellular Signaling Peptides and Proteins, General Medicine, diabetes mellitus, Female, Glomerular Filtration Rate, medicine.medical_specialty, Renal function, wnt5a, Proinflammatory cytokine, 03 medical and health sciences, Insulin resistance, Internal medicine, medicine, Humans, Wnt5a protein, human [supplementary concept], Renal insufficiency, chronic, Anti-inflammatory cytokine, Renal Insufficiency, Chronic, Eye Proteins, Mexico, business.industry, diabetic nephropathy, lcsh:R, Type 2 Diabetes Mellitus, Membrane Proteins, sfrp5, Diabetes mellitus, type 2, Wnt5a, medicine.disease, wnt5a protein, human [supplementary concept], elisa quantification, Cross-Sectional Studies, Diabetes Mellitus, Type 2, Sfrp5 protein, human [supplementary concept], Kidney Failure, Chronic, business, Complication, 030217 neurology & neurosurgery, Kidney disease

وصف الملف: text/html

URL الوصول: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::45398076b771c1a2d50d207f60e22381
https://pubmed.ncbi.nlm.nih.gov/32491086

المؤلفون: Olivera Evrova, Johanna Buschmann

المساهمون: University of Zurich

المصدر: European Cells & Materials, Vol 34, Pp 15-39 (2017)

مصطلحات موضوعية: 0301 basic medicine, VEGF and PDGF (6.2-4.7 ng/mg DDM). Fractionation of growth factors from other DDM components by heparin affinity chromatography diminished osteogenic responses. Depletion of biglycan from DDM also attenuated osteogenic potency, 1303 Biochemistry, lcsh:Diseases of the musculoskeletal system, Angiogenesis, these results demonstrate the potential of DDM for the delivery of physiological levels of growth factors for bone repair processes, Becaplermin, Bioinformatics, with relatively lower concentrations of BMP-2, migration, dentine, 1307 Cell Biology, Tendons, Drug Delivery Systems, FGF, 10266 Clinic for Reconstructive Surgery, decorin, biology, apoptosis, Proto-Oncogene Proteins c-sis, osteoblast differentiation, Drug delivery, the range of bioactive components in dentine and specific biological effects on bone-derived mesenchymal stem cells (MSCs) in humans are less well understood. The aims of this study were to further elucidate the biological response of MSCs to demineralised dentine matrix (DDM) in enhancing wound repair responses and ascertain key contributing components. Dentine was obtained from human teeth and DDM proteins solubilised with ethylenediaminetetraacetic acid (EDTA). Bone marrow derived MSCs were commercially obtained. Cells with a more immature phenotype were then selected by preferential fibronectin adhesion (FN-BMMSCs) for use in subsequent in vitro assays. DDM at 10 µg/mL reduced cell expansion, Platelet-derived growth factor receptor, cell expansion, medicine.drug, Signal Transduction, lcsh:Surgery, 2204 Biomedical Engineering, Context (language use), 610 Medicine & health, Models, Biological, Fibrin, 03 medical and health sciences, In vivo, growth factors, medicine, Animals, Humans, Wound Healing, 1502 Bioengineering, business.industry, 2502 Biomaterials, lcsh:RD1-811, biglycan, In vitro, 030104 developmental biology, attenuated apoptosis and was the minimal concentration capable of inducing osteoblastic differentiation. Enzyme-linked immunosorbent assay (ELISA) quantification of growth factors indicated physiological levels produced the above responses, transforming growth factor β (TGF-β1) was predominant (15.6 ng/mg DDM), biology.protein, Mesenchymal stem cells, lcsh:RC925-935, business, which was partially rescued by the isolated biglycan. Decorin depletion from DDM had no influence on osteogenic potency. Collectively

URL الوصول: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::87896af71997b06e2b0ea7448a8f8326
http://www.ecmjournal.org/papers/vol034/pdf/v034a02.pdf

المؤلفون: Indira Rojo Báez (10445684), Daniel Omar Rivera Castro (10445687), Sarahí Salas Gutiérrez (10445690), Edgar Dehesa López (10445693), Adriana Aguilar Lemarroy (10445696), Luis Felipe Jave Suarez (10445699), Hipólito Castillo Ureta (10445702), Edith Hilario Torres Montoya (10445705), Vicente Olimón Andalón (10445708)

مصطلحات موضوعية: Medicine, Sfrp5 protein, human [supplementary concept], Wnt5a protein, Diabetes mellitus, type 2, Renal insufficiency, chronic, Anti-inflammatory cytokine, Diabetic nephropathy, ELISA quantification, Sfrp5, Wnt5a

Relation: https://figshare.com/articles/dataset/Association_of_serum_levels_of_secreted_frizzled-related_protein_5_and_Wnt_member_5a_with_glomerular_filtration_rate_in_patients_with_type_2_diabetes_mellitus_and_chronic_renal_disease_a_cross-sectional_study/14306309

الاتاحة: https://doi.org/10.6084/m9.figshare.14306309.v1

المؤلفون: Indira Rojo Báez, Daniel Omar Rivera Castro, Sarahí Salas Gutiérrez, Edgar Dehesa López, Adriana Aguilar Lemarroy, Luis Felipe Jave Suarez, Hipólito Castillo Ureta, Edith Hilario Torres Montoya, Vicente Olimón Andalón

المصدر: São Paulo Medical Journal, Vol 138, Iss 2, Pp 133-139

مصطلحات موضوعية: sfrp5 protein, human [supplementary concept], wnt5a protein, human [supplementary concept], diabetes mellitus, type 2, renal insufficiency, chronic, diabetes mellitus, anti-inflammatory cytokine, diabetic nephropathy, elisa quantification, sfrp5, wnt5a, Medicine

وصف الملف: electronic resource

Relation: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-31802020000200133&lng=en&tlng=en; https://doaj.org/toc/1516-3180; https://doaj.org/toc/1806-9460

URL الوصول: https://doaj.org/article/ef9b65fe99d74bc4b722b3f94269ef16