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1Academic Journal
المؤلفون: Marcelo Ehrlich, Eran Bacharach
المصدر: Cancers; Volume 13; Issue 5; Pages: 939
مصطلحات موضوعية: oncolytic viruses, immunoediting, oncogenic signaling, RAS, DNA methyltransferase inhibitor (DNMTi), viral mimicry, epigenetic silencing
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Relation: Cancer Immunology and Immunotherapy; https://dx.doi.org/10.3390/cancers13050939
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2Academic Journal
المؤلفون: Sambathkumar, Rangarajan, Kalo, Eric, Van Rossom, Rob, Faas, Marijke M., de Vos, Paul, Verfaillie, Catherine M.
المصدر: Sambathkumar , R , Kalo , E , Van Rossom , R , Faas , M M , de Vos , P & Verfaillie , C M 2016 , ' Epigenetic Induction of Definitive and Pancreatic Endoderm Cell Fate in Human Fibroblasts ' , Stem cells international , vol. 2016 , 7654321 . https://doi.org/10.1155/2016/7654321
مصطلحات موضوعية: Reprogramming can occur by the introduction of key transcription factors (TFs) as well as by epigenetic changes. We demonstrated, that histone deacetylase inhibitor (HDACi) Trichostatin A (TSA) combined with a chromatin remodeling medium (CRM), induced expression of a number of definitive endoderm and early and late pancreatic marker genes. When CRM was omitted, endoderm/pancreatic marker genes were not induced. Furthermore, treatment with DNA methyltransferase inhibitor (DNMTi), 5-azacytidine (5AZA) CRM did not affect gene expression changes, and when 5AZA was combined with TSA, no further increase, in gene expression of endoderm, pancreatic endoderm, and endocrine markers was seen over levels induced with TSA alone, Interestingly, TSA-CRM did not affect expression of pluripotency and hepatocyte genes but induced some mesoderm transcripts, Upon removal of TSA-CRM, the endoderm/pancreatic gene expression profile returned to baseline. Our findings underscore the, role epigenetic modification in transdifferentiation of one somatic cell into another. However, full reprogramming of fibroblasts to beta-cells will require combination of this approach with TF overexpression and/or culture of the partially reprogrammed cells under beta-cell specific conditions, INSULIN-SECRETING CELLS, PLURIPOTENT STEM-CELLS, BETA-CELLS, IN-VITRO, SMALL MOLECULES, DIFFERENTIATION, TRICHOSTATIN, CONVERSION, CHROMATIN, VIVO
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3
المؤلفون: Eran Bacharach, Marcelo Ehrlich
المصدر: Cancers, Vol 13, Iss 939, p 939 (2021)
Cancersمصطلحات موضوعية: 0301 basic medicine, Cancer Research, medicine.medical_treatment, Review, Biology, lcsh:RC254-282, immunoediting, 03 medical and health sciences, 0302 clinical medicine, Immune system, Immunity, DNA Methyltransferase inhibitor (DNMTi), medicine, Gene silencing, oncogenic signaling, viral mimicry, Immunotherapy, epigenetic silencing, lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens, Oncolytic virus, 030104 developmental biology, Oncology, Immunoediting, Viral replication, oncolytic viruses, 030220 oncology & carcinogenesis, Cancer cell, Cancer research, RAS
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4
المؤلفون: Eric Kalo, Rob Van Rossom, Paul de Vos, Marijke M. Faas, Rangarajan Sambathkumar, Catherine M. Verfaillie
المساهمون: Reproductive Origins of Adult Health and Disease (ROAHD), Translational Immunology Groningen (TRIGR), Man, Biomaterials and Microbes (MBM)
المصدر: Stem Cells International, Vol 2016 (2016)
Stem Cells International
Stem cells international, 2016:7654321. HINDAWI LTDمصطلحات موضوعية: 0301 basic medicine, CHROMATIN, INSULIN-SECRETING CELLS, VIVO, that histone deacetylase inhibitor (HDACi) Trichostatin A (TSA) combined with a chromatin remodeling medium (CRM), treatment with DNA methyltransferase inhibitor (DNMTi), Upon removal of TSA-CRM, role epigenetic modification in transdifferentiation of one somatic cell into another. However, full reprogramming of fibroblasts to beta-cells will require combination of this approach with TF overexpression and/or culture of the partially reprogrammed cells under beta-cell specific conditions, induced expression of a number of definitive endoderm and early and late pancreatic marker genes. When CRM was omitted, no further increase, Histone deacetylase inhibitor, Transdifferentiation, pancreatic endoderm, Cell biology, medicine.anatomical_structure, DIFFERENTIATION, embryonic structures, Reprogramming can occur by the introduction of key transcription factors (TFs) as well as by epigenetic changes. We demonstrated, Endoderm, Reprogramming, PLURIPOTENT STEM-CELLS, medicine.drug, Research Article, lcsh:Internal medicine, animal structures, Article Subject, medicine.drug_class, the endoderm/pancreatic gene expression profile returned to baseline. Our findings underscore the, Interestingly, Biology, SMALL MOLECULES, Chromatin remodeling, 03 medical and health sciences, BETA-CELLS, medicine, Epigenetics, endoderm/pancreatic marker genes were not induced. Furthermore, TRICHOSTATIN, lcsh:RC31-1245, Molecular Biology, and when 5AZA was combined with TSA, and endocrine markers was seen over levels induced with TSA alone, Cell Biology, IN-VITRO, Molecular biology, CONVERSION, 030104 developmental biology, Trichostatin A, TSA-CRM did not affect expression of pluripotency and hepatocyte genes but induced some mesoderm transcripts, 5-azacytidine (5AZA) CRM did not affect gene expression changes, sense organs, in gene expression of endoderm, Definitive endoderm
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