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    Dissertation/ Thesis

    المؤلفون: Sarreshtehdari, Amirhossein

    المساهمون: University/Department: Universitat Pompeu Fabra. Departament de Medicina i Ciències de la Vida

    Thesis Advisors: Burdío Pinilla, Fernando, Moll Sánchez, Xavier, García Sánchez, Tomás

    المصدر: TDX (Tesis Doctorals en Xarxa)

    وصف الملف: application/pdf

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    Academic Journal
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    Academic Journal

    المساهمون: École Centrale de Lyon (ECL), Université de Lyon, Région Auvergne-Rhône Alpes

    المصدر: ISSN: 2731-6319 ; Low-carbon Materials and Green Construction ; https://ec-lyon.hal.science/hal-04845293 ; Low-carbon Materials and Green Construction, 2024, 2 (1), pp.19. ⟨10.1007/s44242-024-00050-w⟩.

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    المساهمون: Spedicati, Mattia, Tivano, Francesca, Zoso, Alice, Bei, Janira, Lavella, Mario, Carmagnola, Irene, Chiono, Valeria

    مصطلحات موضوعية: Human cardiac fibrotic tissues are characterized by a higher stiffness relative to healthy cardiac tissues. These tissues are unable to spontaneously contract and are subjected to passive mechanical stimulation during heart functionality. Moreover, scaffolds that can recapitulate the in vivo mechanical properties of the cardiac fibrotic tissues are lacking. Herein, this study aimed to design and fabricate mechanically stretchable bioartificial scaffolds with biomimetic composition and stiffness comparable to human cardiac fibrotic tissues. Poly(ε-caprolactone) (PCL) scaffolds with a stretchable mesh architecture were initially designed through structural and finite element method (FEM) analyses and subsequently fabricated by melt extrusion additive manufacturing (MEX). Scaffolds were surface functionalized by 3,4-dihydroxy-DL-phenylalanine (DOPA) polymerization (polyDOPA) to improve their interaction with natural polymers. Scaffold pores were then filled with different concentrations (5%, 7%, and 10% w/v) of gelatin methacryloyl (GelMA) hydrogels to support in vitro human cardiac fibroblasts (HCFs) 3D culture, thereby producing bioartificial PCL/GelMA scaffolds. Uniaxial tensile mechanical tests in static and dynamic conditions (1 Hz, 22% maximum strain) demonstrated that the bioartificial scaffolds had in vivo-like stretchability and similar stiffness to that of pathological cardiac tissue (tailored as a function of the number of PCL scaffold layers and GelMA hydrogel concentration). In vitro cell tests on bioartificial scaffolds using HCF-embedded GelMA hydrogels under static conditions displayed increasing cell viability, spreading, and cytoskeleton organization with decreasing GelMA hydrogel concentration. Moreover, α-smooth muscle actin (α-SMA)-positive cells were detected after 7 days of culture in static conditions followed by another 7 days of culture in dynamic conditions and not in HCF-loaded scaffolds cultured in static conditions for 14 days. These results suggested that in vitro culture under cyclic mechanical stimulations could induce an HCF phenotypic switch into myofibroblasts.

    Relation: info:eu-repo/semantics/altIdentifier/wos/WOS:001257555300003; numberofpages:21; journal:INTERNATIONAL JOURNAL OF BIOPRINTING; https://hdl.handle.net/11583/2988744; https://www.accscience.com/journal/IJB/articles/online_first/1438